# US 2014/0193884 A1 · Steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

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- Number: US 2014/0193884 A1
- Document ID: US20140193884A1
- Status: Published application
- Kind: A1
- Application number: 14/205,463
- Filed: 2014-03-12
- Published: 2014-07-10
- Earliest claimed: 2009-02-23
- Assignees: E. I. Du Pont de Nemours and Company
- Applicants: E I Du Pont De Nemours and Company
- Inventors: EDWIN R HENDRICKSON; Abigail K. Luckring; Sharon Jo Keeler; Michael P. Perry; Eric R. Choban
- Web page: https://patentgrep.com/patent/US20140193884A1

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- B09C 1/10: microbiologically, {biologically} or by using enzymes
- C09K 8/582: characterised by the use of bacteria
- C12N 1/205: Bacterial isolates
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12R 2001/065: Azotobacter
- C12R 2001/145: Clostridium
- C12R 2001/38: Pseudomonas
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00)
- C09K: MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

## Claims

### Claim 1

An isolated consortium of microbial species wherein said consortium comprises;
a) at least one first species of the genus Thauera having a 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to SEQ ID NO: 15;
b) at least one second species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs:16, 19, 21, 23, 24-28, 30-41, 67 and 68; and
c) at least one third species having a 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 17, 18, 20, 22, 29, 54, 69 and 86 and combinations thereof.

### Claim 2 (depends on 1)

The isolated consortium of microbial species of claim 1 further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequences selected from the group consisting of SEQ ID NOs: 55, 63, 75, 76 and 81 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 42, 45, 50-52, 64-66 and 79 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 48, 49 and 82.

### Claim 3 (depends on 1)

The isolated consortium of microbial species of claim 1 further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 53, 58 and 87 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 56 and 77.

### Claim 4 (depends on 1)

The isolated consortium of microbial species of claim 1 further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NO: 43.

### Claim 5 (depends on 1)

The isolated consortium of microbial species of claim 1 further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 60-62, 80 and 83 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 20, 44, 46, 57, 70-74, 84 and 85.

### Claim 6 (depends on 1)

The isolated consortium of microbial species of claim 1 further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 47 and 59.

### Claim 7 (depends on 1)

The isolated consortium of microbial species of claim 1 further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NO: 78.