Method for preparing a hydrocarbon
AssigneeShell Oil Company
InventorsThomas Paul HOWARD, Sabine MIDDELHAUFE, Dagmara KOLAK, Stephen J. AVES, John LOVE, David PARKER, George Robert LEE
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Abstract
A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-ACP, or a fatty acyl-CoA or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme.
Claims33
A method for preparing a hydrocarbon comprising:
- obtaining a fatty acid aldehyde by contacting a fatty acid substrate with at least one fatty acid reductase, at least one fatty aldehyde synthetase, and at least one fatty acyl transferase, wherein the fatty acid substrate is selected from the group consisting of a fatty acid, a fatty acyl-ACP, a fatty acyl-CoA and any combination thereof, wherein at least some of said fatty acid substrate is a fatty acyl-ACP;
- obtaining a hydrocarbon by contacting the fatty aldehyde with at least one aldehyde decarbonylase;
- and
- obtaining at least a portion of said fatty acyl-ACP by contacting a keto-acyl-CoA and a malonyl-ACP with at least one 3-ketoacyl-ACP synthase III.
The method of claim 11, wherein the yeast is Saccharomyces cerevisiae and the bacterium is Eschericia coli.
A recombinant host cell comprising a fatty acid reductase and a fatty aldehyde synthetase and a fatty acyl transferase.
Dependencies are inferred from the claim text.