# US 2014/0193871 A1 · Method for enhancing carbon biofixation

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- Number: US 2014/0193871 A1
- Document ID: US20140193871A1
- Status: Published application
- Kind: A1
- Application number: 14/140,627
- Filed: 2013-12-26
- Published: 2014-07-10
- Earliest claimed: 2013-01-04
- Applicants: Industrial Technology Research Institute
- Inventors: Chang-Chieh CHEN; Hsin-Tzu Kuo; Yi-Te Chou; Chin-Chen Hsu; Shao-Wen Wu
- Web page: https://patentgrep.com/patent/US20140193871A1

## CPC

- C12P 7/06: Ethanol, i.e. non-beverage
- C12P 7/065: {with microorganisms other than yeasts}
- C12P 7/16: Butanols
- C12P 7/42: Hydroxy-carboxylic acids
- C12P 7/54: Acetic acid (vinegar C12J)
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

At least one product chosen from acids and alcohols is produced by a microbial fermentation process comprising: providing a gaseous substrate and a liquid medium to a microorganism, wherein the liquid medium comprises lactic acid and/or salts thereof, and wherein the gaseous substrate comprises at least one carbon source chosen from CO and CO2, and allowing the microorganism to produce at least one product chosen from acids and alcohols.

## Claims

### Claim 1

A process for microbial fermentation of a gaseous substrate comprising CO2, the process comprising:
providing a gaseous substrate and a liquid medium to a microorganism, wherein the liquid medium comprises lactic acid and/or salts thereof, and wherein the gaseous substrate comprises CO2, and
allowing the microorganism to produce at least one product chosen from acids and alcohols.

### Claim 2 (depends on 1)

The process according to claim 1, wherein the gaseous substrate further comprises at least one gas chosen from H2, N2, and CO.

### Claim 3 (depends on 1)

The process according to claim 1, wherein the gaseous substrate comprises at least 20% by volume of CO2.

### Claim 4 (depends on 1)

The process according to claim 1, wherein the lactic acid and/or salts thereof is present at a concentration ranging from 0.5 g/L to 3 g/L.

### Claim 5 (depends on 1)

The process according to claim 1, wherein the lactic acid and/or salts thereof is present in an amount sufficient to induce about 1.5 fold or more increase, relative to the microorganism cultured without the presence of lactic acid and/or salts thereof, of at least one gene chosen from codH, codH beta, codH delta, codH gamma, coos1, and metF.

### Claim 6 (depends on 1)

The process according to claim 1, wherein the at least one product is chosen from acetic acid, propionic acid, butyric acid, acrylic acid, and fatty acid.

### Claim 7 (depends on 1)

The process according to claim 1, wherein the at least one product is chosen from ethanol, acetone, propanol, butanol, and 2,3 butanediol.

### Claim 8 (depends on 1)

The process according to claim 1, wherein the microorganism is capable of converting CO2 and/or CO into at least one product chosen from acids and alcohols.

### Claim 9 (depends on 1)

The process according to claim 1, the microorganism is chosen from Clostridium aceticum, Clostridium autoethanogenum, Clostridium carboxidivorans, Clostridium difficile, Clostridium ljungdahli, Moorella thermoacetica (formerly Clostridium thermoaceticum), Methanobacterium thermoautotrophicum, Defulfobacterium autotrophicum, Clostridium sticklandii, Clostridium thermoautotrophicum, Clostridium formicoaceticum, Clostridium magnum, Acetobacterium carbinolicum, Acetobacterium kivui, and Acetobacterium woodii.

### Claim 10

A process of improving efficiency of carbon capture in microbial fermentation, comprising:
providing a gaseous substrate and a liquid medium to a microorganism, wherein the liquid medium comprises lactic acid and/or salts thereof, and wherein the gaseous substrate comprises at least one carbon source chosen from CO and CO2, and allowing the microorganism to produce at least one product chosen from acids and alcohols.

### Claim 11 (depends on 10)

The process according to claim 10, wherein the gaseous substrate further comprises at least one gas chosen from H2 and N2.

### Claim 12 (depends on 10)

The process according to claim 10, wherein the gaseous substrate comprises at least 20% by volume of CO2.

### Claim 13 (depends on 10)

The process according to claim 10, wherein the lactic acid and/or salts thereof is present at a concentration ranging from 0.5 g/L to 3 g/L.

### Claim 14 (depends on 10)

The process according to claim 10, wherein the lactic acid and/or salts thereof is present in an amount sufficient to induce about 1.5 fold or more increase, relative to the microorganism cultured without the presence of lactic acid and/or salts thereof, of at least one gene chosen from codH, codH beta, codH delta, codH gamma, coos1, and metF.

### Claim 15 (depends on 10)

The process according to claim 10, wherein the at least one product is chosen from acetic acid, propionic acid, butyric acid, acrylic acid, and fatty acid.

### Claim 16 (depends on 10)

The process according to claim 10, wherein the at least one product is chosen from ethanol, acetone, propanol, butanol, and 2,3 butanediol.

### Claim 17 (depends on 10)

The process according to claim 10, wherein the microorganism is capable of converting CO2 and/or CO into at least one product chosen from acids and alcohols.

### Claim 18 (depends on 10)

The process according to claim 10, the microorganism is chosen from Clostridium aceticum, Clostridium autoethanogenum, Clostridium carboxidivorans, Clostridium difficile, Clostridium ljungdahli, Moorella thermoacetica (formerly Clostridium thermoaceticum), Methanobacterium thermoautotrophicum, Defulfobacterium autotrophicum, Clostridium sticklandii, Clostridium thermoautotrophicum, Clostridium formicoaceticum, Clostridium magnum, Acetobacterium carbinolicum, Acetobacterium kivui, and Acetobacterium woodii.