Methods Of Producing 7-Carbon Chemicals Via C1 Carbon Chain Elongation Associated With Coenzyme B Synthesis
AssigneeINVISTA North America S.a r.I.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Abstract
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.
Claims38
A method for biosynthesizing a product selected from the group consisting of pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine and 1,7-heptanediol, said method comprising enzymatically synthesizing a seven carbon chain aliphatic backbone from 2-oxoglutarate via three cycles of 2-oxoacid carbon chain elongation and enzymatically forming two terminal functional groups selected from the group consisting of carboxyl, amine, and hydroxyl groups in said backbone, thereby forming the product.
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A recombinant host comprising at least one exogenous nucleic acid encoding (i) a (homo)ncitrate synthase, (ii) a (homo)ncitrate dehydratase and a (homo)naconitate hydratase, (iii) an iso(homo)ncitrate dehydrogenase, and (iv) an indolepyruvate decarboxylase or a 2-oxoglutarate dehydrogenase complex, said host producing pimeloyl-CoA or pimelate semialdehyde.
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Dependencies are inferred from the claim text.