# US 2014/0193855 A1 · Methods and compositions for determining the purity of chemically synthesized nucleic acids

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- Number: US 2014/0193855 A1
- Document ID: US20140193855A1
- Status: Published application
- Kind: A1
- Application number: 14/192,387
- Filed: 2014-02-27
- Published: 2014-07-10
- Earliest claimed: 1999-12-31
- Assignees: Veri-Q, Inc.; North Carolina State University
- Applicants: Veri-Q, Inc.; North Carolina State University
- Inventors: Paul F. Agris; Christopher D.J. Pearce; Lloyd G. Mitchell
- Web page: https://patentgrep.com/patent/US20140193855A1

## CPC

- C07K 16/44: against material not provided for elsewhere {, e.g. haptens, metals, DNA, RNA, amino acids}
- C07K 16/18: against material from animals or humans
- C12Q 1/6804: Nucleic acid analysis using immunogens (immunoassay G01N33/53)
- B01J 2219/00274: Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- Y02P 20/55: Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies.

## Claims

### Claim at position 1

1-25. (canceled)

### Claim 26

A method of making an antibody that specifically binds to a synthetic oligonucleotide having a organic protecting group covalently bound thereto, which antibody does not bind to the said synthetic oligonucleotide when said organic protecting group is not covalently bound thereto, said method comprising the steps of:
synthesizing said synthetic oligonucleotide on a solid particulate support with said organic protecting group covalently bound to said synthetic oligonucleotide, or synthesizing a nucleotide on said solid support with said organic protecting group bound to said nucleotide; and then, without removing said oligonucleotide or nucleotide from said solid support;
immunizing an animal with said synthetic oligonucleotide or nucleotide bound to said solid support in an amount sufficient to produce said antibody.

### Claim 27 (depends on 26)

A method according to claim 26, wherein said synthesizing step is followed by the step of fragmenting said beads prior to said immunizing step.

### Claim 28 (depends on 26)

A method according to claim 26, further comprising the step of:
collecting said antibody from said animal.

### Claim 29 (depends on 26)

A method according to claim 26, further comprising the steps of:
collecting spleen cells from said animal; then
producing a plurality of hybridoma cell lines from said spleen cells; and then
isolating a particular hybridoma cell line that produces said antibody from said plurality of hybridoma cell lines.

### Claim 30 (depends on 26)

A method according to claim 26, wherein said synthetic oligonucleotide is covalently bound to said solid support.

### Claim 31 (depends on 26)

A method according to claim 26, wherein said synthetic oligonucleotide is covalently bound to said solid support with a succinyl linker.

### Claim 32 (depends on 26)

A method according to claim 26, wherein said solid support comprises a controlled pore glass bead.

### Claim at position 9

33-55. (canceled)