# US 2014/0193842 A1 · Non-specific reaction inhibitor, method for inhibiting non-specific reaction, and kit

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- Number: US 2014/0193842 A1
- Document ID: US20140193842A1
- Status: Published application
- Kind: A1
- Application number: 14/129,510
- Filed: 2012-06-28
- Published: 2014-07-10
- Earliest claimed: 2011-06-29
- Assignees: Mitsubishi Chemical Medience Corporation
- Inventors: Yoshikazu Okamura; Shigeru Tashiro
- Web page: https://patentgrep.com/patent/US20140193842A1

## CPC

- G01N 33/5306: {Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding}
- C07D 207/416: with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to other ring carbon atoms
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)

## Abstract

Provided is a non-specific reaction inhibitor for achieving the accurate detection and quantitation of a trace component (a target substance) contained in a sample, in an immunoassay, by simply and effectively inhibiting a non-specific reaction associated with the measurement. The non-specific reaction inhibitor comprises a substance of the formula I: wherein R1 and R2 together form a double bond between carbons, to which they are respectively bonded directly, or R1 is a hydrogen atom and R2 is a group formed by removing H from an SH-group-containing compound, B is a support, and L is a spacer arm portion.

## Claims

### Claim 1

A non-specific reaction inhibitor for an immunoassay, comprising a substance of the formula I:
wherein R1 and R2 together form a double bond between carbons to which they are respectively bonded directly, or R1 is a hydrogen atom and R2 is a group formed by removing H from an SH-group-containing compound, B is a support, and L is a spacer arm portion.

### Claim 2 (depends on 1)

The non-specific reaction inhibitor according to claim 1, wherein the support is a carrier protein or a solid phase carrier.

### Claim 3 (depends on 2)

The non-specific reaction inhibitor according to claim 2, wherein the carrier protein is albumin.

### Claim 4 (depends on 2)

The non-specific reaction inhibitor according to claim 2, wherein the solid phase carrier is a group of particles.

### Claim 5 (depends on 1)

An immunoassay using the non-specific reaction inhibitor according to claim 1.

### Claim 6 (depends on 5)

The immunoassay according to claim 5, wherein the immunoassay is a latex agglutination optical measurement method, an enzyme immunoassay, a nephelometric immunoassay, an enzyme-linked immunosorbent assay, a fluorescence immunoassay, or a radioimmunoassay.

### Claim 7 (depends on 5)

The immunoassay according to claim 5, wherein a sample is reacted with the non-specific reaction inhibitor, before an immunological reaction specific for a target substance containing the sample is carried out.

### Claim 8 (depends on 5)

The immunoassay according to claim 5, wherein an immunological reaction specific for a target substance containing a sample is carried out in the presence of the non-specific reaction inhibitor.

### Claim 9 (depends on 5)

The immunoassay according to claim 5, wherein the immunoassay is a measurement by a sandwich method, and one or more steps of immunological reactions specific for a target substance containing a sample is carried out in the presence of the non-specific reaction inhibitor.

### Claim 10 (depends on 1)

An immunoassay kit comprising the non-specific reaction inhibitor according to claim 1.