# US 2014/0193809 A1 · Methods for genetic analysis of dna to detect sequence variances

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- Number: US 2014/0193809 A1
- Document ID: US20140193809A1
- Status: Published application
- Kind: A1
- Application number: 14/073,779
- Filed: 2013-11-06
- Published: 2014-07-10
- Earliest claimed: 2000-05-23
- Inventors: Jeffrey Olson; Vincent P. Stanton, JR.
- Web page: https://patentgrep.com/patent/US20140193809A1

## CPC

- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q 1/6858: Allele-specific amplification
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Methods for determining genotypes and haplotypes of genes are described. Also described are single nucleotide polymorphisms and haplotypes in the ApoE gene and methods of using that information.

## Claims

### Claim 1

A method of detecting the presence of a variance in a target nucleic acid sequence, comprising
amplifying a template nucleic acid sequence to generate an amplification product, wherein said amplifying results in the insertion of a bifunctional restriction enzyme recognition site into said amplification product; and
analyzing said amplification product.

### Claim at position 2

-10. (canceled)