# US 2014/0193803 A1 · Methods, compositions, and kits for determining hepatitus a virus

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- Number: US 2014/0193803 A1
- Document ID: US20140193803A1
- Status: Published application
- Kind: A1
- Application number: 14/126,269
- Filed: 2012-08-01
- Published: 2014-07-10
- Earliest claimed: 2011-09-07
- Assignees: Grifols Therapeutics Inc.
- Inventors: Danuta Wronska; Brett Buno; Stefan Burde
- Web page: https://patentgrep.com/patent/US20140193803A1

## CPC

- C12Q 1/706: {for hepatitis}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to compositions, methods, and kits for determining the presence or absence of HAV in a sample.

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a nucleotide sequence, or a complement thereof, as set forth in:
(SEQ ID NO: 1)
5′-GCG CCC GGC GGG GTC AAC TCC AT-3′;
(SEQ ID NO: 2)
5′-AGC CAA GTT AAC ACT GCA AGG-3′;
or
(SEQ ID NO: 3)
5′-TTA GCA TGG AGC TGT AGG AGT CTA AAT TGG GG-3′.

### Claim 2

A composition comprising a pair of oligonucleotide primers comprising a forward primer having a sequence as set forth in SEQ ID NO: l and a reverse primer having a sequence as set forth in SEQ ID NO:2, wherein the pair of primers are capable of annealing to a target sequence under a PCR condition to amplify the target sequence.

### Claim 3 (depends on 2)

The composition of claim 2 further comprising an oligonucleotide probe having the sequence as set forth in SEQ ID NO:3, wherein the probe is capable of annealing to the target sequence.

### Claim 4

A method for amplifying a target sequence, the method comprising:
performing a PCR with the target sequence as template, wherein performing comprises providing the PCR with a forward primer comprising the sequence as set forth in SEQ ID NO: l and a reverse primer comprising the sequence as set forth in SEQ ID NO:2.

### Claim 5 (depends on 4)

The method of claim 4, wherein the target sequence corresponds to a cDNA prepared from RNA of a sample comprising an HAV.

### Claim 6 (depends on 5)

The method of claim 5 further comprising:
detecting an amplicon generated by the forward and the reverse primer, wherein the presence of the amplicon determines whether the HAV is present in the sample.

### Claim 7 (depends on 6)

The method of claim 6, wherein detecting comprises providing a polynucleotide probe to the PCR whereby the amplicon, if present, contacts the probe, wherein the probe comprises the sequence as set forth in SEQ ID NO:3 or a complement thereof.

### Claim 8 (depends on 7)

The method of claim 7, wherein the probe is labeled with FAM/BHQ-1.

### Claim 9 (depends on 1)

A kit comprising one or more of the isolated nucleic acid molecules of claim 1.