# US 2014/0193505 A1 · Biodegradable carrier with adjustable zeta potentials and particle sizes, method for making the same, and pharmaceutical composition comprising the same

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- Number: US 2014/0193505 A1
- Document ID: US20140193505A1
- Status: Published application
- Kind: A1
- Application number: 13/923,699
- Filed: 2013-06-21
- Published: 2014-07-10
- Earliest claimed: 2013-01-09
- Assignees: National Cheng Kung University
- Applicants: National Cheng Kung University
- Inventors: YEE-SHIN LIN; YU-HUNG CHEN
- Web page: https://patentgrep.com/patent/US20140193505A1

## CPC

- A61K 39/12: Viral antigens
- A61K 39/385: Haptens or antigens, bound to carriers
- A61P 31/14: for RNA viruses
- A61P 37/04: Immunostimulants
- A61K 47/34: Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers (A61K47/10 takes precedence)
- A61K 47/36: Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K 2039/55555: {Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers}
- A61K 2039/55583: {Polysaccharides}
- A61K 2039/6087: {Polysaccharides; Lipopolysaccharides [LPS]}
- A61K 2039/6093: {Synthetic polymers, e.g. polyethyleneglycol [PEG], Polymers or copolymers of (D) glutamate and (D) lysine}
- C12N 2770/24134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention is related to a biodegradable carrier with adjustable zeta potentials and particle sizes, a method for making the same, and a pharmaceutical composition comprising the same. In such a method, a first solution comprising a first biodegradable macromolecule is prepared, and a second solution comprising a second biodegradable macromolecule is also prepared according to a desired zeta potential of a biodegradable carrier and further added into the first solution to form a mixture solution. The biodegradable carrier with the desired zeta potentials is formed by the attraction force between the different electric properties. Then, the mole number of the first biodegradable macromolecule and the second biodegradable macromolecule in the mixture solution are proportionally adjusted according to a desired particle size of the biodegradable carrier. Therefore, the zeta potential and the particle size of the biodegradable carrier are adjustable artificially.

## Claims

### Claim 1

A method for making a biodegradable carrier with adjustable zeta potentials and particle sizes comprising the steps of:
preparing a first solution comprising a first biodegradable macromolecule with a first electric property;
preparing a second solution comprising a second biodegradable macromolecule with a second electric property according to a desired zeta potential of a biodegradable carrier and adding the second solution into the first solution to form a mixture solution, wherein the first electric property is opposite to the second electric property for making the biodegradable carrier with the desired zeta potential by attraction force between the different electric properties;
proportionally adjusting the mole number of the first biodegradable macromolecule and the second biodegradable macromolecule in the mixture solution according to a desired particle size of the biodegradable carrier, wherein the particle size of the biodegradable carrier is positively correlated with the mole number of a solute in the mixture solution for making the biodegradable carrier with the desired particle size; and
filtrating the mixture solution after the proportionally adjusting step to obtain the biodegradable carrier.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the first macromolecule is heparin or polyglutamic acid, and the first electric property is negatively charged.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the second macromolecule is chitosan or collagen, and the second electric property is positively charged.

### Claim 4 (depends on 1)

The method as claimed in claim 1, further comprising a step of:
adding a carried substance into the first solution or the second solution for making the carried substance be held in the biodegradable carrier.

### Claim 5 (depends on 4)

The method as claimed in claim 4, wherein the carried substance is selected from the group consisting of nucleic acid, short peptide, protein drugs, small molecular compounds, viruses, bacterium, and cells.

### Claim 6 (depends on 5)

The method as claimed in claim 5, wherein the electric property of the carried substance is the same as that of solution which the carried substance is added into.

### Claim 7 (depends on 1)

A biodegradable carrier with adjustable zeta potentials and particle sizes obtained by the method as claimed in claim 1.

### Claim 8 (depends on 7)

The biodegradable carrier as claimed in claim 7, wherein the particle size of the biodegradable carrier ranges from micrometers to nanometers.

### Claim 9 (depends on 8)

The biodegradable carrier as claimed in claim 8, wherein the particle size of the biodegradable carrier ranges from 10 μm to 40 nm.

### Claim 10 (depends on 7)

The biodegradable carrier as claimed in claim 7, wherein the zeta potential of the biodegradable carrier ranges from +35 mv to −35 mv.

### Claim 11 (depends on 7)

A pharmaceutical composition comprising:
a biodegradable carrier as claimed in claim 7;
a carried substance held in the biodegradable carrier; and
at least one pharmaceutically acceptable vehicle.

### Claim 12 (depends on 11)

The pharmaceutical composition as claimed in claim 11, wherein the carried substance is selected from the group consisting of nucleic acid, short peptide, protein drugs, small molecular compounds, viruses, bacterium, and cells.

### Claim 13 (depends on 11)

The pharmaceutical composition as claimed in claim 11, which is used for producing a vaccine or a drug for treating or preventing hemorrhagic dengue fever or dengue shock syndrome.