# US 2014/0193461 A1 · Streptococcus vaccine compositions and methods of using the same

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- Number: US 2014/0193461 A1
- Document ID: US20140193461A1
- Status: Published application
- Kind: A1
- Application number: 14/202,620
- Filed: 2014-03-10
- Published: 2014-07-10
- Earliest claimed: 2009-05-14
- Assignees: The Regents of the University of Michigan
- Applicants: The Regents of the University of Michigan
- Inventors: James R. Baker, JR.; Paul E. Makidon; Whitney A. Dunlap; Jessica A. Knowlton; Benjamin Swanson
- Web page: https://patentgrep.com/patent/US20140193461A1

## CPC

- A61K 39/092: {Streptococcus}
- A61P 31/04: Antibacterial agents
- A61K 2039/521: {inactivated (killed)}
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention provides methods and compositions for the stimulation of immune responses. In particular, the present invention provides immunogenic nanoemulsion compositions and methods of using the same for the induction of immune responses (e.g., innate and/or adaptive immune responses (e.g., for generation of host immunity against a bacterial species of the genus Streptococcus (e.g., Streptococcus pneumoniae))). Compositions and methods of the present invention find use in, among other things, clinical (e.g. therapeutic and preventative medicine (e.g., vaccination)) and research applications.

## Claims

### Claim 1

An immunogenic composition comprising a nanoemulsion and an immunogen, wherein said nanoemulsion comprises a non-ionic surfactant, ethanol, cetylpyridinium chloride (CPC), oil and water, and wherein said immunogen comprises killed whole cell Streptococcus pneumoniae.

### Claim 2 (depends on 1)

The immunogenic composition of claim 1, wherein the composition comprises between 106 and 108 CFU of killed Streptococcus pneumonia.

### Claim 3 (depends on 1)

The immunogenic composition of claim 1, wherein the composition comprises 106 CFU of killed Streptococcus pneumonia.

### Claim 4 (depends on 1)

The immunogenic composition of claim 1, wherein the composition comprises 108 CFU of killed Streptococcus pneumonia.

### Claim 5 (depends on 1)

The immunogenic composition of claim 1, wherein the Streptococcus pneumoniae cells are killed via mixing with said nanoemulsion.

### Claim 6 (depends on 1)

The immunogenic composition of claim 1, wherein the Streptococcus pneumoniae cells are killed via mixing with ethanol.

### Claim 7

An immunogenic composition comprising a nanoemulsion and a Streptococcus pneumoniae immunogen, wherein said nanoemulsion comprises a non-ionic surfactant, ethanol, cetylpyridinium chloride (CPC), oil and water.

### Claim 8 (depends on 7)

The immunogenic composition of claim 7, wherein the Streptococcus pneumoniae immunogen is selected from the group consisting of Streptococcus pneumoniae polysaccharide, Streptococcus pneumoniae protein, killed whole cell Streptococcus pneumonia, and a combination thereof.