# US 2014/0193456 A1 · Method for Drying-Conservation of Natural Substances

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- Number: US 2014/0193456 A1
- Document ID: US20140193456A1
- Status: Published application
- Kind: A1
- Application number: 14/150,648
- Filed: 2014-01-08
- Published: 2014-07-10
- Earliest claimed: 2013-01-09
- Inventors: Hristem Mitkov Dyanov; Paoula Hristemova Dyanova
- Web page: https://patentgrep.com/patent/US20140193456A1

## CPC

- A61K 36/09: Lichens
- A01N 1/162: Temperature processes, e.g. following predefined temperature changes over time
- A61K 36/00: Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines {(antigens from pollen A61K39/36)}
- A61K 36/02: Algae
- A61K 36/06: Fungi, e.g. yeasts
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The invention comprises of an implementation of the lyophilization process in a method for a large-scale preservation of the entire biological material prior to the extraction and/or the utilization/manipulation of any substances of the biological content. The drying-conservation of all the natural substances is achieved via deep-freezing evaporation followed by complete drying of the biological material by the means of sublimation, and other additional manipulations and procedures suitable for the preparation of various commercially viable products further on.

## Claims

### Claim 1

A method for obtaining biological material and substances followed by commercially viable preparation and preservation of plants, funguses, lichens and algae, entire plant-, fungus-, lichen- or algae material, parts of entire plant-, fungus-, lichen- or algae species and/or any desired combinations of these species or parts of them, or mixes of them including but not limited to those serving herbal, medicinal and supplemental properties and purposes, with sad method comprising of the following steps, manipulations and conditions listed below in a non-successive order:
rapid instantaneous deep freezing of the entire plant-, fungus-, lichen- and/or algae material used for commercial purposes shortly after the material collection, within minutes (less than two hours, but preferably within 20 minutes or less), before the vitality of the cells is destroyed,—by the means of using (preferably but not limited to) liquefied and/or solidified deep-freezing liquids or gases to momentarily submerse the biological material with their deep-cold solid-, liquid- or gaseous state (molecules) in certain enclosed volumes resulting in instant rapid deep freezing of the biological material to temperatures below minus−30 degree Celsius (° C.), and by the means of using preferably but not limited to liquid nitrogen (at minus−180° C. to minus−170° C.), dry ice (carbon dioxide) (at minus−80° C. to minus−30° C.) or else (below minus−30° C.)—thereby preserving the prevailing majority of both the cell structure and content as in the state of vitality mainly as a result of so called water “glassification” (vitrification) process (i.e. water freezing without crystallization, volume increase, and morphological and chemical structure disruption of the biomaterial);
storing, transporting, grinding or else treating the sad plant-, fungus-, lichen- and/or algae material and/or mixing different species materials or parts of them under conditions preferably (but not limited to) being submerged in liquid nitrogen, dry ice or otherwise at temperatures not increasing above the minus−20° C. until the containing water is completely evaporated from the biological material by sublimation (from solid—to gaseous form) and removed from the volume (vessel) containing the dry biological material;
drying the frozen (below the minus−20° C.) biological material entirely by sublimation of the containing water molecules from the biological material into the surrounding air followed by further removal of the water vapor out of the bio-material-containing volume (vessel) by means including but not limited to removal by vacuumization, adsorption into hydroscopic substrates (materials), ventilation and liquefaction (condensation) on acceptor surfaces & materials, etc. and/or combination of the above;
mechanical processing of the biological material in enclosed volumes under completely dry condition maintained by vacuum, chemically inert gases such as but not limited to nitrogen and/or else atmosphere—such a processing including but not limited to chopping, grinding, weighting, transferring, storage-vessel filling, capsule filling, tablet making, encapsulation within hard-surface materials capable of maintaining further dry conditions, preventing contacts with volatile solutions, gases and/or else under cold- or ambient temperatures not increasing above 60° C.

### Claim 2 (depends on 1)

A method of claim 1 comprising a step of rapid instantaneous deep freezing of the entire plant-, fungus-, lichen- and/or algae material used for commercial purposes shortly after the material collection, within minutes (less than two hours, but preferably within 20 minutes or less), before the vitality of the cells is destroyed,—by the means of submersing the bio-material using frozen hydrocarbons and their mixes with or without liquefied and/or solidified gases or else (such as but not limited to chemically inert deep-frozen liquids or gases, instant and direct contact to deep-frozen molecules, chunks and/or surfaces, etc.), and/or by direct and instant contact of the biological material to pre-cooled surfaces providing instant cooling of the biological material to temperatures below minus−30 degree Celsius (° C.)—thereby preserving the prevailing majority of both the cell structure and content as in the state of vitality mainly as a result of so called water “glassification” (vitrification) process (i.e. water freezing without crystallization, volume increase, and morphological and chemical structure disruption of the biomaterial).

### Claim 3 (depends on 1)

A method described in claim 1 combined or not with additional procedures of collecting (in instance or in selective order) all the co-evaporated and co-separated biological substances that originate from the processed plant-, fungus-, lichen- and/or algae material (including but not limited to aromatic and/or light-molecular-weight oils, pheromones, etc.) and that remain together with the water vapor removed from the biological material due to the technical impossibility to be separated from the water vapor; by the sub-sequential removal of these bio-substances from the water evaporate via all appropriate physical and chemical means, their biophysical isolation and/or condensation, and re-introduction into the volume containing the dry biological material—where such an re-introduction is optional and/or desired for the final product preparation in cases of particular applications but may not always be obligatory option.

### Claim 4 (depends on 1)

Storing the biological material obtained by the method described in claim 1 under dry conditions into enclosed volumes (vessels, containers, bags, capsules etc.) ensuring a dry storage and/or (preferably) an absence of chemically harmful gases such as but not limited to oxygen, volatile chemical elements, molecules and vapors; thereby the storage volumes and conditions should maintain chemically inert (preferably but not limited to vacuum- or nitrogen-) atmosphere, or any transitional conditions between the last mentioned (vacuum, nitrogen and/or other chemically inert gases or combinations of—and transitions between them) while handling the material of biological origin.

### Claim 5 (depends on 1)

A method described in claim 1 for commercial preparation and preservation of plants, funguses, lichens and/or algae, entire plant-, fungus-, lichen- and/or algae material, parts of the plant-, fungus-, lichen- and/or algae species and/or any desired combinations of different biological species or parts of them, including but not limited to those serving herbal, medicinal and supplemental properties and purposes, with sad method serving for the preparation of commercially viable substances and mixes of plant- and/or fungal origin rather than intended for laboratory and/or research usage.

### Claim 6 (depends on 1)

A method described in claim 1 for commercial preparation and preservation of plants, funguses, lichens and/or algae, entire plant-, fungus-, lichen- and/or algae material, parts of the plant-, fungus-, lichen- and/or algae species and/or any desired combinations of different biological species or parts of them with such a method serving biochemical and biophysical conditions for total and complete, biological and chemical conservation and preservation of the entire plant- and/or fungal content—practically identical to those existing in the nature before the vitality state is halted and/or destroyed—and within the prevailing majority of the biological material allowing for maintaining the vitality-potential conditions of the cells, cell structures and cellular content (within the mechanically processed biological material) that are finally prepared for commercial usage and/or market introduction—without a special introduction of antifreeze compounds for the purpose of vitality stabilization on any stage of the biomaterial processing.

### Claim 7 (depends on 1)

A method described in claim 1 for commercial preparation and preservation of plants, funguses, lichens and/or algae, entire plant-, fungus-, lichen- and/or algae material, parts of entire plant-, fungus-, lichen- and/or algae species and/or any desired combinations of different biological species or parts of them, which method allows for post-conservational (after-freezing) sub-sequential manipulation of the biological (plant-, fungus-, lichen- and/or algae-) material for the extraction and/or separation, or other manipulation of the biological material with the intention but not limited to isolation, purification or else usage and utilization of any particular or combinative biological component(s) of the biological material intended for commercial, medicinal and/or supplemental purposes rather than for any laboratory research investigations prior to the final dried-product preparation.

### Claim 8 (depends on 1)

The commercial product manipulation according to claim 1 which is frozen and sheared simultaneously and/or successively in a vessel comprising a rotor with attached shear blades with the rotor attached to a motor wherein said rotor comprises an impeller of correct proportions to scrape the surface near the edge of the vessel as the impeller rotates under ambient or frozen conditions thereby successfully removing any bio-material deposits from the surface of the vessel—in the presence or absence of inert atmospheric conditions.

### Claim 9 (depends on 1)

The commercial product according to claim 1 is frozen and sheared in a device vessel (chamber) equipped with rotating blade(s) and double walls designed to maintain the presence and re-filling of a deep-freeze coolant such as liquid nitrogen, dry carbon dioxide, liquid hydrocarbons and/or silicones—inside the shredding chamber or between the double walls of the vessel chamber.

### Claim 10

A method for commercial preparation and preservation of plants, funguses, lichens and/or algae, entire plant-, fungus-, lichen- and/or algae material, parts of entire plant-, fungus-, lichen- and/or algae species and/or any desired combinations of different biological species or parts of them, including but not limited to those serving herbal, medicinal and/or supplemental properties and purposes, during the sad method the entire process of processing the plant and/or fungal material from it's obtaining to it's inclusion (packing) in market-oriented vessels (encapsulation) and commercial shipment maintaining the following conditions: temperatures below minus−20° C. (preferably, between minus−180 to minus−40 degree Celsius) if residual water is present within the plant material or temperatures below +60° C. (preferably, within a temperature maximum of +15 to +30° C.)—in the absence of water (dry state), and preferably maintaining vacuum or chemically inert atmosphere of gases and/or else vapor (preferable but not limited to nitrogen) for minimizing the oxidation and/or other chemical alteration.