# US 2014/0193429 A1 · Metal-citrate transporter antigen from streptomyces coelicolor and uses thereof

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- Number: US 2014/0193429 A1
- Document ID: US20140193429A1
- Status: Published application
- Kind: A1
- Application number: 14/216,110
- Filed: 2014-03-17
- Published: 2014-07-10
- Earliest claimed: 2008-06-06
- Assignees: Syracuse University
- Inventors: Robert P. Doyle; Joshua J. Lensbouer
- Web page: https://patentgrep.com/patent/US20140193429A1

## CPC

- C07K 16/1292: {from Actinomyces; from Streptomyces (G)}
- A61P 31/04: Antibacterial agents
- A61P 31/06: for tuberculosis
- A61P 37/04: Immunostimulants
- C07K 14/36: from Actinomyces; from Streptomyces (G)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to an isolated antigen from Streptomyces coelicolor that is useful for developing, inter alia, vaccines against pathogenic bacteria of humans and animals. The present invention also relates to vaccines and antibodies developed using the isolated antigen. The present invention also relates to methods of using the antigen, vaccines, and antibodies of the present invention to detect, treat, and prevent infection and diseases associated with pathogenic bacteria.

## Claims

### Claim 1

An isolated antibody or antigen-binding fragment thereof that specifically binds with high affinity to an antigen, wherein said antigen comprises:
(a) an isolated metal-citrate transporter from Streptomyces coelicolor; or
(b) an isolated immunogenic polypeptide fragment of said metal-citrate transporter, wherein said immunogenic fragment retains an antigenic activity of said metal-citrate transporter.

### Claim 2 (depends on 1)

The antibody according to claim 1, wherein said antibody is suitable for oral, parenteral, intravenous, subcutaneous, intranasal, or aerosolized administration in a human or animal.

### Claim 3 (depends on 1)

The antibody according to claim 1, wherein said antibody is a polyclonal antibody or a monoclonal antibody.

### Claim 4 (depends on 2)

The antibody according to claim 2, wherein the monoclonal antibody is of a type selected from the group consisting of a human monoclonal antibody, a chimeric monoclonal antibody, a murine monoclonal antibody, and a humanized monoclonal antibody.

### Claim 5 (depends on 2)

The antibody according to claim 2, wherein the monoclonal antibody is a single chain monoclonal antibody.

### Claim 6 (depends on 1)

A pharmaceutical composition comprising:
the antibody according to claim 1; and
a pharmaceutically acceptable carrier.

### Claim 7 (depends on 6)

The pharmaceutical composition according to claim 6 further comprising a physiologically acceptable antibiotic.

### Claim 8 (depends on 1)

An isolated antisera containing the antibody according to claim 1.

### Claim 9 (depends on 1)

A diagnostic kit comprising the antibody according to claim 1 and a detecting agent for detecting binding by that antibody.

### Claim 10 (depends on 9)

The diagnostic kit according to claim 9, wherein said detecting agent comprises a detectable label that is linked to said antibody.