# US 2014/0193391 A1 · Method And Compositions For Improving Selective Catabolysis And Viability In Cells Of Keratin Surfaces

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- Number: US 2014/0193391 A1
- Document ID: US20140193391A1
- Status: Published application
- Kind: A1
- Application number: 14/142,212
- Filed: 2013-12-27
- Published: 2014-07-10
- Earliest claimed: 2013-01-07
- Assignees: ELC Management LLC
- Applicants: ELC Management LLC
- Inventors: Nadine A. Pernodet; Donald F. Collins; Dawn Layman; Daniel B. Yarosh
- Web page: https://patentgrep.com/patent/US20140193391A1

## CPC

- A61K 8/66: Enzymes
- A61K 8/645: {Proteins of vegetable origin; Derivatives or degradation products thereof}
- A61K 8/733: {Alginic acid; Salts thereof}
- A61K 8/9717: Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
- A61K 8/9728: Fungi, e.g. yeasts
- A61K 8/9789: Magnoliopsida [dicotyledons]
- A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria
- A61P 17/00: Drugs for dermatological disorders
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A61Q 19/00: Preparations for care of the skin
- A61Q 19/08: Anti-ageing preparations
- A61K 2800/10: General cosmetic use
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

A composition for treating keratin surfaces to stimulate selective catabolysis and improve cellular viability comprising at least one autophagy activator and at least one DNA repair enzyme, and a method for improving selective catabolysis and cellular viability by treating with the composition.

## Claims

### Claim 1

A topical composition comprising at least one cellular autophagy activator and at least one DNA repair enzyme.

### Claim 2 (depends on 1)

The composition of claim 1 wherein the cells are keratinocytes or fibroblasts.

### Claim 3 (depends on 1)

The composition of claim 1 wherein autophagy activator comprises a yeast extract.

### Claim 4 (depends on 1)

The composition of claim 1 wherein the autophagy activator comprises a yeast extract selected from the group consisting of those from genuses Lithothamnium, Melilot, Citrus, Candida, Lens, Urtica, Carambola, Momordica, Yarrowia, Plumbago, or mixtures thereof.

### Claim 5 (depends on 1)

The composition of claim 1 wherein the autophagy activator is selected from the group consisting of Lithothamniumn calcaneum, Melilotus officinalis, Citrus limonum, Candida saitoana, Lens culinaria, Urtica dioica, Averrhoa carambola, Momordica charantia, Yarrowia lipolytica, Plumbago zeylanica.

### Claim 6 (depends on 1)

The composition of claim 1 wherein the autophagy activator is a stimulator of one or more of: (a) MAP-LC3; (b) ATG5-12; (c) protein p53; (d) AMPK; or (e) DRAM.

### Claim 7 (depends on 5)

The composition of claim 5 wherein the autophagy activator is an inhibitor of mTOR.

### Claim 8 (depends on 1)

The composition of claim 1 wherein the DNA repair enzyme is selected from the group consisting of base excision repair (BER) enzymes, nucleotide excision repair (NER) enzymes, DNA polymerases, DNA helicases, Mismatch repair (MMR) enzymes, and mixtures thereof.

### Claim 9 (depends on 8)

The composition of claim 8 wherein the DNA repair enzyme is selected from the group consisting of Arabidopsis Thaliana extract either alone or in admixture with lecithin and water; Lactobacillus ferment, Micrococcus lysate, Plankton extract, Bifida ferment lysate; and mixtures thereof.

### Claim 10 (depends on 5)

The composition of claim 5 wherein the autophagy activator is yeast extract is from the genus Candida and the DNA repair enzyme comprises Arabidopsis thaliana extract either alone or in admixture with lecithin and water.

### Claim 11 (depends on 1)

The composition of claim 1 additionally comprising at least one proteasome activator.

### Claim 12 (depends on 11)

The composition of claim 11 wherein the proteasome activator is algin, alginate, or hydrolyzed algin.

### Claim 13 (depends on 1)

The composition of claim 1 additionally comprising a CLOCK or PER1 gene activator comprising:
(a) a peptide having from about 3 to 13 amino acid residues and of the formula (I) (SEQ ID No. 1):
R1-(AA)n-X1-S-T-P-X2-(AA)p-R2, wherein
X1 represents threonine, serine, or is equal to zero,
X2 represents an isoleucine, leucine, proline, valine, alanine, glycine, or is equal to zero,
AA represents any amino acid or derivative thereof, and n and p are whole numbers between 0 and 4,
R1 represents the primary amine function of the N-terminal amino acid, either free or substituted by a protective grouping that may be chosen from either an acetyl group, a benzoyl group, a tosyl group, or a benzyloxycarbonyl group,
R2 represents the hydroxyl group of the carboxyl function of the C-terminal amino acid, which may be substituted by a protective grouping that may be chosen from either a C1 to C20 alkyl chain or an NH2, NHY, or NYY group with Y representing a C1 to C4 alkyl chain, and
wherein said formula (I) sequence may contain substitutions of amino acids X1 and X2 with other chemically equivalent amino acids;
(b) a peptide having the amino acid sequence Ser-Pro-Leu-Gln-NH2;
(c) cichoric acid; and
(d) mixtures thereof.

### Claim 14 (depends on 13)

The composition of claim 13 wherein the CLOCK or PER1 gene activators are selected from the group consisting of:
S-T-P-NH2
Ser-Thr-Pro-NH2
(SEQ ID No. 2)
Y-V-S-T-P-Y-N-NH2
Tyr-Val-Ser-Thr-Pro-Tyr-Asn-NH2
(SEQ ID No. 3)
NH2-V-S-T-P-E-NH2
NH2-Val-Ser-Thr-Pro-Glu-NH2
(SEQ ID No. 4)
NH2-L-H-S-T-P-P-NH2
NH2-Leu-His-Ser-Thr-Pro-Pro-NH2
(SEQ ID No. 5)
CH3NH-R-H-S-T-P-E-NH2
CH3-NH-Arg-His-Ser-Thr-Pro-Glu-NH2
(SEQ ID No. 6)
CH3NH-H-S-T-P-E-CH3NH
CH3-NH-His-Ser-Thr-Pro-Glu-CH3-NH2
and mixtures thereof.

### Claim 15 (depends on 13)

The composition of claim 13 wherein the CLOCK or PER1 gene activators are selected from:
S-T-P-NH2
Ser-Thr-Pro-NH2
(SEQ ID No. 4)
NH2-L-H-S-T-P-P-NH2
NH2-Leu-His-Ser-Thr-Pro-Pro-NH2
and mixtures thereof.

### Claim 16 (depends on 1)

The composition of claim 1 additionally comprising a CLOCK or PER1 gene activator selected from the group consisting of Tripeptide-32, Tetrapeptide-26 or mixtures thereof.

### Claim 17

A method for increasing cellular viability and selective catabolysis in cells of keratin surfaces in need of such treatment comprising applying to the surface a composition comprising at least one autophagy activator and at least one DNA repair enzyme.

### Claim 18 (depends on 17)

The method of claim 17 wherein the cellular viability and selective autophagy activation results in improved cellular detoxification.

### Claim 19 (depends on 17)

The method of claim 17 wherein the composition is applied at night prior to evening rest.

### Claim 20 (depends on 17)

The method of claim 17 wherein the keratin surfaces are skin, hair, or nails.