# US 2014/0193377 A1 · Preparation of parental cell bank from foetal tissue

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- Number: US 2014/0193377 A1
- Document ID: US20140193377A1
- Status: Published application
- Kind: A1
- Application number: 14/131,736
- Filed: 2012-07-10
- Published: 2014-07-10
- Earliest claimed: 2011-07-11
- Assignees: Centre Hospitalier Universitaire Voudois (CHUV)
- Inventors: Lee Ann Laurent-Applegate
- Web page: https://patentgrep.com/patent/US20140193377A1

## CPC

- C12N 5/0655: {Chondrocytes; Cartilage}
- A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
- A61K 35/33: Fibroblasts
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61P 19/04: for non-specific disorders of the connective tissue
- A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease
- C12N 5/0603: {Embryonic cells (production of embryos, nuclear transfer A01K67/027); Embryoid bodies}
- C12N 5/066: {Tenocytes; Tendons, Ligaments}
- G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics}
- G01N 2500/10: involving cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.

## Claims

### Claim 1

An in vitro non-enzymatic method for isolation, expansion and development of foetal cells selected from the group consisting of foetal epiphyseal chondrocytes, foetal Achilles tenocytes or foetal skin fibroblasts, comprising the steps of:
a) using foetal sample selected from ulnar foetal cartilage comprising foetal epiphyseal chondrocyte; foetal Achilles tendon comprising foetal Achilles tenocytes; or foetal abdominal skin comprising foetal skin fibroblasts;
b) micro-dissecting and dispersing said ulnar foetal cartilage, foetal Achilles tendon or foetal abdominal skin sample by mechanical attachment to scalpel scored surface;
c) culturing said ulnar foetal cartilage, foetal Achilles tendon or foetal abdominal skin sample in vitro under conditions wherein said foetal epiphyseal chondrocytes, foetal Achilles tenocytes or foetal abdominal skin fibroblasts proliferate,
d) selecting and isolating first adherent foetal epiphyseal chondrocyte cell populations, first adherent foetal Achilles tenocyte cell populations and first adherent foetal skin fibroblast populations therefrom.

### Claim 2 (depends on 1)

The method of claim 1, wherein ulnar foetal cartilage sample is a sample of foetal proximal ulnar epiphysis.

### Claim 3 (depends on 1)

Foetal epiphyseal chondrocyte (FEC) cell line obtained by the method of claim 1, having the designation FE002-Cart and deposited under accession number ECACC 12070303-FE002-Cart.

### Claim 4 (depends on 1)

Foetal Achilles tenocyte cell line obtained by the method of claim 1, having the designation FE002-Ten and deposited under accession number ECACC 12070302-FE002-Ten.

### Claim 5 (depends on 1)

Foetal skin fibroblast cell line obtained by the method of claim 1, having the designation FE002-SK2 and deposited under accession number ECACC 12070301-SK2.

### Claim 6 (depends on 3)

Use of the foetal epiphyseal chondrocyte (FEC) of claim 3 for the production of new cartilage tissue and/or three dimensional constructs.

### Claim 7 (depends on 4)

Use of the foetal Achilles tenocytes of claim 4 for the production of new tendon tissue and/or three dimensional constructs.

### Claim 8 (depends on 5)

Use of the foetal skin fibroblasts of claim 5 for the production of new skin tissue and/or three dimensional constructs.

### Claim at position 9

9-11. (canceled)

### Claim 12 (depends on 3)

A method for repair and regeneration of osteochondral tissue and musculoskeletal tissue comprising administering to a subject in need thereof the foetal epiphyseal chondrocyte (FEC) of claim 3.

### Claim 13 (depends on 4)

A method for repair and regeneration of tendon tissue and musculoskeletal tissue comprising administering to a subject in need thereof the foetal Achilles tenocytes of claim 4.

### Claim 14 (depends on 5)

A method for repair and regeneration of skin tissue and for treating burns, wounds and fibrotic condition comprising administering to a subject in need thereof the foetal skin fibroblasts of claim 5.

### Claim 15 (depends on 3)

A method for treating osteochondral diseases or defects, arthritis and musculoskeletal diseases comprising administering to a subject in need thereof the foetal epiphyseal chondrocyte (FEC) of claim 3.

### Claim 16 (depends on 4)

A method for treating musculoskeletal diseases and tendonopathies comprising administering to a subject in need thereof the foetal Achilles tenocytes of claim 4.

### Claim 17 (depends on 5)

A method for treating skin diseases comprising administering to a subject in need thereof the foetal skin fibroblasts of claim 5.

### Claim 18 (depends on 3)

A screening method for development of therapeutic agents and/or medical devices for the treatment of arthritic, osteochondral defects, and musculoskeletal tissue repair, comprising the use of foetal epiphyseal chondrocyte (FEC) of claim 3.

### Claim 19 (depends on 4)

A screening method for development of therapeutic agents and/or medical devices for the treatment of cartilage repair, tendon repair and musculoskeletal tissue repair, comprising the use of foetal Achilles tenocytes of claim 4.

### Claim 20 (depends on 5)

A screening method for development of therapeutic agents and/or medical devices for the treatment of skin repair, comprising the use of foetal skin fibroblasts of claim 5.