# US 2014/0193374 A1 · Methods and compositions for culturing cells > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0193374 A1 - Document ID: US20140193374A1 - Status: Published application - Kind: A1 - Application number: 14/099,769 - Filed: 2013-12-06 - Published: 2014-07-10 - Earliest claimed: 2012-12-07 - Assignees: Arteriocyte Inc. - Applicants: Arteriocyte Inc. - Inventors: Yukang Zhao; Stephen Fischer; Anirudhasingh Sodha - Web page: https://patentgrep.com/patent/US20140193374A1 ## CPC - C12N 5/0647: {Haematopoietic stem cells; Uncommitted or multipotent progenitors} - C12M 23/14: {Bags} - C12M 23/20: {Material Coatings (immunocoatings C12M25/00)} - C12M 25/16: {Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00)} - C12N 2533/30: Synthetic polymers - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract The invention provides, among other things, methods and compositions for expanding stem cells. The invention further provides methods, devices and systems for directing differentiation of expanded stem cells. The invention further provides methods, devices and systems for treating a subject with differentiated cells in a subject in need thereof. ## Claims ### Claim 1 A device for culturing cells, comprising polymeric nanofibers bonded to the interior surface of a container. ### Claim 2 (depends on 1) The device of claim 1, wherein the device has a flexible surface. ### Claim 3 (depends on 1) The device of claim 1, wherein the device has a rigid surface. ### Claim 4 (depends on 1) The device of claim 1, wherein the device has at least one inlet/outlet valve. ### Claim 5 (depends on 1) The device of claim 1, wherein the device further comprises polymeric nanofibers that are not bonded to the surface of the container. ### Claim 6 (depends on 1) The device of claim 1, wherein the device is a culture bag. ### Claim 7 (depends on 1) The device of claim 1, wherein the surface of the container comprises synthetic polymers. ### Claim 8 (depends on 7) The device of claim 7, wherein the synthetic polymers are polystyrene. ### Claim 9 (depends on 1) The device of claim 1, wherein the device is thermally sealed. ### Claim 10 (depends on 1) The device of claim 1, wherein the device is water tight. ### Claim 11 (depends on 1) The device of claim 1, wherein the device comprises a material that has a high rate of gas exchange. ### Claim 12 (depends on 1) The device of claim 1, wherein the device comprises a material that has a low rate of gas exchange. ### Claim 13 (depends on 1) The device of claim 1, wherein the device comprises at least one polymer. ### Claim 14 (depends on 13) The device of claim 13, wherein the polymer is selected from the group consisting of: polystyrene, fluorinated ethylene propylene (FEP), ethylene vinyl acetate (EVA) and EVO. ### Claim 15 (depends on 1) The device of claim 1, wherein the polymeric nanofibers are bonded directly to the interior surface of the container. ### Claim 16 (depends on 1) The device of claim 1, wherein the polymeric nanofibers are bonded to the interior surface of the container using mechanical means. ### Claim 17 (depends on 1) The device of claim 1, wherein the polymeric nanofibers are bonded to at least one substrate that is bonded to the interior surface of the container. ### Claim 18 (depends on 17) The device of claim 17, wherein the substrate is selected from the group consisting of: a poly-acrylate, a poly-acetate and a polyester. ### Claim 19 (depends on 18) The device of claim 18, wherein the substrate is selected from the group consisting of: PMMA, EVA and PET. ### Claim 20 (depends on 1) The device of claim 1, wherein the polymeric nanofibers are random nanofiber meshes or films. ### Claim 21 (depends on 1) The device of claim 1, wherein said polymeric nanofibers are aligned nanofiber meshes or films. ### Claim 22 (depends on 1) The device of claim 1, wherein said polymeric nanofibers are polyethersulfone (PES) meshes or films. ### Claim 23 (depends on 1) The device of claim 1, wherein said polymeric nanofibers are surface-conjugated with functional groups. ### Claim 24 (depends on 23) The device of claim 23, wherein said functional groups are selected from the group consisting of: hydroxyl, carboxyl, and amino groups. ### Claim 25 (depends on 1) The device of claim 1, wherein said polymeric nanofibers are surface-conjugated with fibronectin. ### Claim 26 (depends on 1) The device of claim 1, wherein the cells are stem cells. ### Claim 27 (depends on 1) The device of claim 1, wherein the cells are CD34+ cells. ### Claim 28 (depends on 1) The device of claim 1, wherein the cells are CD133+ cells. ### Claim 29 A bioreactor system, comprising polymeric nanofibers bonded to the interior surface of a container and cells. ### Claim at position 30 30-32. (canceled) ### Claim 33 A method for expanding stem cells, comprising providing cells and culturing said cells in a culture container comprising polymeric nanofibers bonded to the interior surface. ### Claim at position 32 34-64. (canceled) ### Claim 65 A method of expanding stem cells, comprising: (a) providing a population of cells including stem cells, (b) purifying stem cells from the population, (c) providing a bioreactor system containing polymeric nanofibers, and (d) culturing said stem cells in said bioreactor. ### Claim at position 34 66-80. (canceled)