# US 2014/0193311 A1 · Integrated high throughput system for the analysis of biomolecules

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- Number: US 2014/0193311 A1
- Document ID: US20140193311A1
- Status: Published application
- Kind: A1
- Application number: 14/071,494
- Filed: 2013-11-04
- Published: 2014-07-10
- Earliest claimed: 2001-01-18
- Assignees: Intrinsic Bioprobes, Inc.
- Applicants: Intrinsic Bioprobes, Inc.
- Inventors: Kemmons Tubbs; Karl F. Gruber; Randall W. Nelson
- Web page: https://patentgrep.com/patent/US20140193311A1

## CPC

- B01J 20/286: Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J 20/28097: {being coated, filled or plugged with specific compounds}
- B01J 20/281: Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J 20/32: Impregnating or coating {; Solid sorbent compositions obtained from processes involving impregnating or coating}
- B01J 20/3204: {Inorganic carriers, supports or substrates}
- B01J 20/3208: {Polymeric carriers, supports or substrates}
- B01J 20/3242: {Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group}
- B01J 20/3259: {comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulfur with at least one silicon atom}
- B01J 20/3265: {with an organic functional group containing a metal, e.g. a metal affinity ligand}
- B01J 20/3272: {Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds}
- B01J 20/3274: {Proteins, nucleic acids, polysaccharides, antibodies or antigens}
- B01J 20/3282: {Crosslinked polymers}
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- G01N 30/6095: {Micromachined or nanomachined, e.g. micro- or nanosize}
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- G01N 33/545: Synthetic resin
- G01N 33/6803: {General methods of protein analysis not limited to specific proteins or families of proteins}
- G01N 33/6845: {Methods of identifying protein-protein interactions in protein mixtures}
- H01J 49/164: {Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418)}
- H01J 49/40: Time-of-flight spectrometers (H01J49/36 takes precedence)
- B01J 2220/54: Sorbents specially adapted for analytical or investigative chromatography
- B01J 2220/64: In a syringe, pipette, e.g. tip or in a tube, e.g. test-tube or u-shape tube
- G01N 30/02: Column chromatography
- G01N 2570/00: Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
- Y10T 436/255: including use of a solid sorbent, semipermeable membrane, or liquid extraction
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

## Claims

### Claim 1

An affinity microcolumn comprising a polymer contained within a housing and affinity reagents bound to the polymer, wherein the affinity reagents are either activated or activatable.

### Claim 2 (depends on 1)

The affinity microcolumn of claim 1 wherein the polymer is formed by molding.

### Claim 3 (depends on 1)

The affinity microcolumn of claim 1 wherein the polymer is exposed to at least one of chemical etching and electro etching.

### Claim 4 (depends on 1)

The affinity microcolumn of claim 1 wherein the affinity reagents that are bound to the polymer further comprise affinity receptors bound to the affinity reagents.

### Claim 5 (depends on 4)

The affinity microcolumn of claim 4 further comprising a tethering molecule that is activated or activatible and binds the affinity receptors to the affinity reagents.

### Claim 6 (depends on 4)

The affinity microcolumn of claim 4 further comprising an amplification media that is activated or activatible and is interposed between the affinity reagents and the affinity receptors, where the amplification media allows a high density of affinity receptors to be bound to the affinity reagents than in the absence of the amplification media.

### Claim 7 (depends on 4)

The affinity microcolumn of claim 4 further comprising an amplification media interposed between the affinity reagents and the affinity receptors, where the amplification media allows better access by an analyte to the affinity receptors than in the absence of the amplification media.

### Claim 8 (depends on 6)

The affinity microcolumn of claim 6 wherein the amplification media comprises at least one of a biological polymer, a non-biological organic polymer, and an inorganic polymer.

### Claim 9 (depends on 7)

The affinity microcolumn of claim 7 wherein the amplification media comprises at least one of a biological polymer, a non-biological organic polymer, and an inorganic polymer.

### Claim 10 (depends on 1)

The affinity microcolumn of claim 1 wherein the housing is a micropipette.

### Claim 11 (depends on 10)

The affinity microcolumn of claim 10 wherein the housing further comprises at least one projection that supports the polymer.

### Claim 12 (depends on 1)

The affinity microcolumn of claim 1 wherein the housing is a manifold having more than one microchannel, the polymer fitting into at least one of the microchannels.