# US 2014/0193302 A1 · Base material comprising hydrophilic layer > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0193302 A1 - Document ID: US20140193302A1 - Status: Published application - Kind: A1 - Application number: 14/130,047 - Filed: 2012-04-16 - Published: 2014-07-10 - Earliest claimed: 2011-06-28 - Assignees: Dai Nippon Printing Co., Ltd. - Inventors: Norihiko Ookouchi; Hirohito Ayame; Yuichi Tanaka; Hiroko Watanabe - Web page: https://patentgrep.com/patent/US20140193302A1 ## CPC - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract This invention provides a base material comprising a plastic-containing support and, on its surface, a hydrophilic layer comprising an ethylene glycol chain (an EG chain) composed of one or more ethylene glycol units, as well as a method for producing such base material. A polysiloxane-containing primer layer is provided on the support comprising a plastic material on its surface, and the EG chain is covalently bound to a polysiloxane side chain of the primer layer. Thus, a hydrophilic layer comprising the EG chain can be provided on the surface of the plastic-containing support. ## Claims ### Claim 1 A base material comprising at least: a support containing a plastic material on its surface; a polysiloxane-containing primer layer on the surface of the support; and on the primer layer, a hydrophilic layer containing an ethylene glycol chain composed of one or more ethylene glycol units, which is covalently bound to a side chain of the polysiloxane of the primer layer. ### Claim 2 (depends on 1) The base material according to claim 1, wherein the primer layer is bound to the surface of the support by physical adsorption. ### Claim 3 (depends on 2) The base material according to claim 2, wherein the plastic material is at least one member selected from the group consisting of polystyrene, polypropylene, polyvinyl chloride, polyethylene, cyclic polyolefin, acrylic resin, and polyethylene terephthalate. ### Claim 4 (depends on 1) The base material according to claim 1, wherein one end of the ethylene glycol chain is covalently bound to the side chain of the polysiloxane of the primer layer and the other end of the ethylene glycol chain is bound to a functional group capable of forming a covalent bond with another substance. ### Claim 5 (depends on 4) The base material according to claim 4, wherein the functional group comprises a functional group having n number of nitrogen atoms and the nitrogen concentration in the hydrophilic layer is from 0.010 to 0.050×n, provided that the carbon concentration resulting from C—O bonds in the hydrophilic layer is 1. ### Claim 6 (depends on 1) The base material according to claim 1, wherein one end of the ethylene glycol chain is covalently bound to the side chain of the polysiloxane of the primer layer and the other end of the ethylene glycol chain is sealed with a hydroxyl group. ### Claim 7 (depends on 1) A method for producing the base material according to claim 1 comprising: a step of forming a primer layer on the surface of the support comprising polymerizing a silanol compound containing an organic group having a carbon atom directly bound to a silicon atom and a functional group, so as to form a polysiloxane-containing primer layer; and a step of forming a hydrophilic layer comprising allowing the primer layer to react with ethylene glycol or polyethylene glycol through a reaction between a hydroxyl group of the ethylene glycol or polyethylene glycol and a functional group on a side chain of the polysiloxane which corresponds to the functional group of the silanol compound or is derived from the functional group of the silanol compound, so as to form a covalent bond between the ethylene glycol chain composed of one or more ethylene glycol units and the side chain. ### Claim 8 (depends on 7) The method according to claim 7, wherein the step of forming a primer layer comprises: a step of hydrolyzing a silicon compound that generates the silanol compound via hydrolysis, so as to generate the silanol compound; and a step of bringing a solution comprising the silanol compound and a base dissolved in an alcohol into contact with the surface of the support. ### Claim 9 (depends on 8) The method according to claim 8, wherein the silicon compound is 3-glycidoxypropyltrimethoxysilane or 3-glycidoxypropyltriethoxysilane. ### Claim 10 (depends on 7) The method according to claim 7, which further comprises a step of binding a functional group capable of forming a covalent bond with another substance to an end of the ethylene glycol chain that is not bound to the polysiloxane, following the step of forming a hydrophilic layer, wherein the step of binding a functional group comprises a step of bringing a solution comprising a precursor compound that imparts the functional group through the reaction with a hydroxyl group of the ethylene glycol chain dissolved in substantially anhydrous acetonitrile or dimethyl sulfoxide or a solvent mixture thereof into contact with the hydrophilic layer at 4° C. to 25° C. ### Claim 11 (depends on 10) The method according to claim 10, wherein the functional group is (1H-imidazol-1-yl)carbonyl group and the precursor compound is 1,1′-carbonyldiimidazole, or the functional group is succinimidyloxycarbonyl group and the precursor compound is di(N-succinimidyl)carbonate.