# US 2014/0191186 A1 · Regenerative Nanosensor Devices

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- Number: US 2014/0191186 A1
- Document ID: US20140191186A1
- Status: Published application
- Kind: A1
- Application number: 14/147,095
- Filed: 2014-01-03
- Published: 2014-07-10
- Earliest claimed: 2013-01-04
- Assignees: Yale University
- Applicants: Yale University
- Inventors: Mark A. Reed; Xuexin Duan; Nitin Rajan
- Web page: https://patentgrep.com/patent/US20140191186A1

## CPC

- G01N 27/4145: {specially adapted for biomolecules, e.g. gate electrode with immobilised receptors}
- G01N 27/4146: {involving nanosized elements, e.g. nanotubes, nanowires}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention provides a regenerative nanosensor device for the detection of one or more analytes of interest. In certain embodiments, the device comprises a nanostructure having a reversible functionalized coating comprising a supramolecular assembly. Controllable and selective disruption of the assembly promotes desorption of at least part of the reversible functionalized coating thereby allowing for reuse of the regenerative device.

## Claims

### Claim 1

A regenerative nanosensor device comprising a nanostructure forming an electrically conducting pathway between at least a first contact and a second contact, the nanostructure surface having a reversible coating comprising a reversible functionalized supramolecular assembly.

### Claim 2 (depends on 1)

The device of claim 1 wherein the nanostructure is fabricated on a substrate.

### Claim 3 (depends on 2)

The device of claim 2, wherein the substrate is selected from the group consisting of a semiconductor substrate and a semiconductor-on-insulator (SOI) substrate.

### Claim 4 (depends on 1)

The device of claim 1, wherein the nanostructure is selected from the group consisting of a nanowire, a nanoribbon, and a carbon nanotube.

### Claim 5 (depends on 1)

The device of claim 1, wherein the first and second contacts form a source and a drain contact, respectively.

### Claim 6 (depends on 1)

The device of claim 1, wherein the device further comprises at least one solution chamber positioned atop at least a portion of the nanostructure such that the contents of the solution chamber are accessible to the nanostructure, and wherein the device further comprises at least one inlet and at least one outlet for delivering a fluid sample to the solution chamber.

### Claim 7 (depends on 1)

The device of claim 1, wherein the coating comprises a self-assembled monolayer (SAM) atop of the nanostructure surface.

### Claim 8 (depends on 7)

The device of claim 7, wherein the coating comprises a linker layer bound to the SAM.

### Claim 9 (depends on 7)

The device of claim 7, wherein the SAM comprises β-cyclodextrin (β-CD), thereby providing a β-CD SAM.

### Claim 10 (depends on 7)

The device of claim 7, wherein the SAM comprises a polyelectrolyte film.

### Claim 11 (depends on 8)

The device of claim 8, wherein the linker layer comprises a linker comprising a receptor molecule which specifically binds to an analyte of interest.

### Claim 12 (depends on 11)

The device of claim 11, wherein the linker comprises a chain region which prevents non-specific binding to the nanostructure.

### Claim 13 (depends on 11)

The device of claim 11, wherein the linker comprises a guest moiety which reversibly binds to the SAM.

### Claim 14 (depends on 12)

The device of claim 12, wherein the chain region comprises oligo(ethylene glycol) (OEG).

### Claim 15 (depends on 13)

The device of claim 13, wherein the guest moiety comprises adamantane.

### Claim 16

A method of manufacturing a regenerative nanosensor device comprising:
providing a device comprising a nanostructure forming an electrically conducting pathway between at least a first contact and a second contact; and
forming a reversible coating comprising a reversible functionalized supramolecular assembly atop the surface of the nanostructure.

### Claim 17 (depends on 16)

The method of claim 16, wherein the nanostructure is fabricated on a substrate.

### Claim 18 (depends on 17)

The method of claim 17, wherein the substrate is selected from the group consisting of a semiconductor substrate and a semiconductor-on-insulator (SOI) substrate.

### Claim 19 (depends on 16)

The method of claim 16, wherein the nanostructure is selected from the group consisting of a nanowire, a nanoribbon, and a carbon nanotube.

### Claim 20 (depends on 16)

The method of claim 16, wherein the first and second contacts form a source and a drain contact, respectively.

### Claim 21 (depends on 16)

The method of claim 16, wherein forming the reversible coating comprises applying a self-assembled monolayer (SAM) atop the surface of a nanostructure.

### Claim 22 (depends on 21)

The method of claim 21, wherein the method comprises contacting the nanostructure with β-cyclodextrin (β-CD), thereby providing a β-CD SAM.

### Claim 23 (depends on 21)

The method of claim 21, wherein the SAM comprises a polyelectrolyte thin film.

### Claim 24 (depends on 21)

The method of claim 21, wherein forming the reversible coating further comprises forming a linker layer atop the SAM.

### Claim 25 (depends on 24)

The method of claim 24, wherein forming the reversible coating further comprises contacting the SAM with a linker comprising a receptor molecule and a guest moiety, wherein the guest moiety reversibly binds to the SAM.

### Claim 26 (depends on 22)

The method of claim 22, wherein forming the reversible coating further comprises forming a linker layer atop the β-CD SAM by contacting the β-CD SAM with a linker comprising a receptor molecule and a guest moiety comprising adamantane, wherein the guest moiety reversibly binds to the β-CD SAM.

### Claim 27

A method of replacing a receptor molecule functionalized on the nanostructure of a regenerative nanosensor device comprising:
providing a regenerative nanosensor device comprising a nanostructure forming an electrically conducting pathway between at least a first contact and a second contact, the nanostructure surface having a first coating comprising a reversible functionalized supramolecular assembly comprising a first receptor molecule; and
disrupting the supramolecular assembly of the first coating to induce the selective removal of at least one layer of the first coating, thereby forming an unfunctionalized nanostructure.

### Claim 28 (depends on 27)

The method of claim 27 comprising coating the unfunctionalized nanostructure with a second coating comprising a reversible functionalized supramolecular assembly comprising a second receptor molecule.

### Claim 29 (depends on 27)

The method of claim 27, wherein the coating comprises a SAM.

### Claim 30 (depends on 29)

The method of claim 29, wherein the SAM comprises O-cyclodextrin (β-CD), thereby providing a β-CD SAM.

### Claim 31 (depends on 27)

The method of claim 27, wherein the coating comprises a linker layer comprising a linker comprising a receptor molecule.

### Claim 32 (depends on 31)

The method of claim 31, wherein the linker comprises a chain region which prevents non-specific binding to the nanostructure.

### Claim 33 (depends on 31)

The method of claim 31, wherein the linker comprises a guest moiety which reversibly binds to the SAM.

### Claim 34 (depends on 32)

The method of claim 32, wherein the chain region comprises oligo(ethylene glycol) (OEG).

### Claim 35 (depends on 33)

The method of claim 33, wherein the guest moiety comprises adamantane.

### Claim 36 (depends on 30)

The method of claim 30, wherein disrupting the supramolecular assembly of the first coating comprises administering a solution to the device, wherein the solution comprises β-CD to induce the desorption of the linker from the SAM.

### Claim 37 (depends on 29)

The method of claim 29, wherein the SAM comprises a polyelectrolyte thin film.

### Claim 38 (depends on 37)

The method of claim 37, wherein disrupting the supramolecular assembly of the first coating comprises altering the pH of a solution surrounding the nanostructure to induce the desorption of the polyelectrolyte thin film.