# US 2014/0190894 A1 · Device And Method Incorporating A Slideable Lid For Extracting A Targeted Fraction From A Sample

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- Number: US 2014/0190894 A1
- Document ID: US20140190894A1
- Status: Published application
- Kind: A1
- Application number: 13/737,448
- Filed: 2013-01-09
- Published: 2014-07-10
- Earliest claimed: 2013-01-09
- Assignees: Wisconsin Alumni Research Foundation
- Applicants: Wisconsin Alumni Research Foundation
- Inventors: David J. Beebe; Ben P. Casavant; David J. Guckenberger; Scott M. Berry
- Web page: https://patentgrep.com/patent/US20140190894A1

## CPC

- G01N 1/34: Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- B01L 3/502792: {for moving individual droplets on a plate, e.g. by locally altering surface tension}
- B01L 3/5088: {confining liquids at a location by surface tension, e.g. virtual wells on plates, wires (B01L3/50857 takes precedence)}
- B03C 1/00: Magnetic separation
- B03C 1/02: acting directly on the substance being separated
- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- C12N 15/1013: {by using magnetic beads}
- B01L 2200/0668: Trapping microscopic beads
- B01L 2300/045: whereby the whole cover is slidable
- B01L 2300/0803: Disc shape
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2400/043: magnetic forces
- B01L 2400/0644: rotary valves
- B01L 2400/065: sliding valves
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A device and a method for isolating a target from a biological sample are provided. The target is bound to solid phase substrate to form target bound solid phase substrate. The device includes a lower plate with an upper surface having a plurality of regions. The biological sample is receivable on a first of the regions. An upper plate has a lower surface directed to the upper surface of the lower plate. A force is positioned adjacent the upper plate and attracts the target bound solid phase substrate toward the lower surface of the upper plate. At least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample.

## Claims

### Claim 1

A device for isolating a target from a biological sample, the target being bound to solid phase substrate to form target bound solid phase substrate, comprising:
a lower plate including an upper surface having a plurality of regions, the biological sample receivable on a first of the regions;
an upper plate having a lower surface directed to the upper surface of the lower plate;
a force adjacent the upper plate, the force attracting the target bound solid phase substrate toward the lower surface of the upper plate;
wherein at least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample.

### Claim 2 (depends on 1)

The device of claim 1 wherein the regions of the lower plate are hydrophilic.

### Claim 3 (depends on 1)

The device of claim 1 wherein portions of the upper surface of the outside of the regions of the lower plate are hydrophobic.

### Claim 4 (depends on 1)

The device of claim 1 wherein the lower surface of the upper plate is hydrophobic.

### Claim 5 (depends on 1)

The device of claim 1 wherein the upper plate is axially movable between the first and second positions.

### Claim 6 (depends on 1)

The device of claim 1 wherein the upper plate is rotatable between the first and second positions.

### Claim 7 (depends on 1)

The device of claim 1 wherein the upper surface of the lower plate and lower surface of the upper surface are spaced by a predetermined distance.

### Claim 8

A method for isolating a target from a biological sample, the target being bound to solid phase substrate to form target bound solid phase substrate, comprising the steps of:
providing the biological sample at a region of a surface of a lower plate;
positioning an upper plate in spaced relation to the lower plate, the upper plate having a lower surface directed to the upper surface of the lower plate;
drawing the target bound solid phase substrate toward the lower surface of the upper plate with a force; and
moving at least one of the lower plate and the upper plate from a first position wherein the target bound solid phase substrate in the biological sample are drawn toward the lower surface of the upper plate to a second position wherein the target bound solid phase substrate are isolated from the biological sample.

### Claim 9 (depends on 8)

The method of claim 8 wherein the region of the upper surface of the lower plate is hydrophilic.

### Claim 10 (depends on 8)

The method of claim 8 wherein the region of the upper surface of the lower plate is a first region and wherein the upper surface of the lower plate includes a plurality of regions that are hydrophilic.

### Claim 11 (depends on 10)

The method of claim 10 wherein portions of the upper surface of the lower surface outside of the regions are hydrophobic.

### Claim 12 (depends on 8)

The method of claim 8 wherein the lower surface of the upper plate is hydrophobic.

### Claim 13 (depends on 9)

The method of claim 9 wherein the upper plate moves along a longitudinal axis between the first and second positions.

### Claim 14 (depends on 9)

The method of claim 9 wherein the upper plate is rotatable between the first and second positions.

### Claim 15 (depends on 9)

The method of claim 9 comprising an additional step of spacing the upper surface of the lower plate and lower surface of the upper surface by a predetermined distance.

### Claim 16

A method for isolating a target from a biological sample, the target being bound to solid phase substrate to form target bound solid phase substrate, comprising the steps of:
providing the biological sample at a first region of a surface of a first plate;
depositing a fluid on a second region of the surface of the first plate;
positioning a second plate in spaced relation to the first plate, the second plate having a hydrophobic surface directed towards the surface of the first plate;
drawing the target bound solid phase substrate toward the surface of the second plate with a force; and
moving at least one of the first plate and the second plate from a first position wherein the target bound solid phase substrate in the biological sample are drawn toward the surface of the second plate to a second position wherein the target bound solid phase substrate are isolated from the biological sample.

### Claim 17 (depends on 16)

The method of claim 16 wherein portions of the surface of the first plate outside of the first and second regions are hydrophobic.

### Claim 18 (depends on 16)

The method of claim 16 wherein the second plate moves along a longitudinal axis between the first and second positions.

### Claim 19 (depends on 16)

The method of claim 16 wherein the second plate is rotatable between the first and second positions.

### Claim 20 (depends on 16)

The method of claim 16 comprising the additional step of spacing the surface of the second plate from the surface of the first plate by a predetermined distance.

### Claim 21 (depends on 16)

The method of claim 16 wherein the force is magnetic.

### Claim 22 (depends on 16)

The method of claim 16 wherein the target bound solid phase substrate are received in the fluid with the at least one of the first plate and the second plate in the second position.

### Claim 23 (depends on 22)

The method of claim 22 comprising the additional step of isolating the target bound solid phase substrate from the force.