# US 2014/0190243 A1 · Fluorescence spectrophotometer

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- Number: US 2014/0190243 A1
- Document ID: US20140190243A1
- Status: Published application
- Kind: A1
- Application number: 13/980,354
- Filed: 2011-09-30
- Published: 2014-07-10
- Earliest claimed: 2011-09-30
- Assignees: Shimadzu Corporation
- Inventors: Masahide Gunji
- Web page: https://patentgrep.com/patent/US20140190243A1

## CPC

- G01J 3/4406: {Fluorescence spectrometry}
- G01J 3/06: Scanning arrangements {arrangements for order-selection}
- G01J 3/18: using diffraction elements, e.g. grating (gratings per se G02B)
- G01J 3/42: Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry (beam switching arrangements G01J3/08)
- G01N 21/64: Fluorescence; Phosphorescence
- G01N 30/74: Optical detectors {(measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J)}
- G01N 2021/6417: {Spectrofluorimetric devices}
- G01N 2021/6419: {Excitation at two or more wavelengths}
- G01N 2021/6421: {Measuring at two or more wavelengths}
- G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A fluorescence spectrophotometer according to the present invention includes: a light source 1; a sample cell 3; an excitation-side light-dispersing system 2 for dispersing a light from the light source 1 and for casting a desired wavelength of light into the sample cell 3; an emission-side light-dispersing system 4 for dispersing a light emitted from the sample cell 3, the emission-side light-dispersing system 4 being located off an optical path of a transmitted light exiting from the sample cell 3 after being cast from the excitation-side light-dispersing system 2 into the sample cell 3; and a photodetector 5 capable of detecting, among the light from the emission-side light-dispersing system 4, an emission light having the same wavelength as the light cast from the excitation-side light-dispersing system 2 into the sample cell 3.

## Claims

### Claim 1

A fluorescence spectrophotometer, comprising:
a light source;
a sample cell;
an excitation-side light-dispersing system for dispersing a light from the light source and for casting a desired wavelength of light into the sample cell;
an emission-side light-dispersing system for dispersing a light emitted from the sample cell, the emission-side light-dispersing system being located off an optical path of a transmitted light exiting from the sample cell after being cast from the excitation-side light-dispersing system into the sample cell; and
a photodetector capable of detecting, among the light from the emission-side light-dispersing system, an emission light having a same wavelength as the light cast from the excitation-side light-dispersing system into the sample cell.

### Claim 2 (depends on 1)

The fluorescence spectrophotometer according to claim 1, comprising:
a controller for continuously varying a set wavelength of the excitation-side light-dispersing system and a set wavelength of the emission-side light-dispersing system over a predetermined wavelength range while keeping the two wavelengths equal to each other; and
a spectrum data acquirer for obtaining spectrum data from detection results obtained with the photodetector for each of the set wavelengths of the excitation-side light-dispersing system and the emission-side light-dispersing system.

### Claim 3

A liquid chromatograph system, comprising a fluorescence spectrophotometer and a separation column, the fluorescence spectrophotometer including:
a light source;
a sample cell;
an excitation-side light-dispersing system for dispersing a light from the light source and for casting a desired wavelength of light into the sample cell;
an emission-side light-dispersing system for dispersing a light emitted from the sample cell, the emission-side light-dispersing system being located off an optical path of a transmitted light exiting from the sample cell after being cast from the excitation-side light-dispersing system into the sample cell;
a photodetector capable of detecting, among the light from the emission-side light-dispersing system, an emission light having a same wavelength as the light cast from the excitation-side light-dispersing system into the sample cell;
a controller for continuously varying a set wavelength of the excitation-side light-dispersing system and a set wavelength of the emission-side light-dispersing system over a predetermined wavelength range while keeping the two wavelengths equal to each other; and
a spectrum data acquirer for obtaining spectrum data from detection results obtained with the photodetector for each of the set wavelengths of the excitation-side light-dispersing system and the emission-side light-dispersing system, wherein:
a sample separated into components by the separation column is passed through the sample cell together with a mobile phase; and
the spectrum data acquirer obtains data on a temporal change of a spectrum for each of the set wavelengths.

### Claim 4

A method for detecting an excitation wavelength in a fluorescence spectrophotometer having:
a light source;
a sample cell;
an excitation-side light-dispersing system for dispersing a light from the light source and for casting a desired wavelength of light into the sample cell;
an emission-side light-dispersing system for dispersing a light emitted from the sample cell, the emission-side light-dispersing system being located off an optical path of a transmitted light exiting from the sample cell after being cast from the excitation-side light-dispersing system into the sample cell; and
a photodetector capable of detecting, among the light from the emission-side light-dispersing system, an emission light having a same wavelength as the light cast from the excitation-side light-dispersing system into the sample cell, the method comprising steps of:
continuously varying a set wavelength of the excitation-side light-dispersing system and a set wavelength of the emission-side light-dispersing system over a predetermined wavelength range while keeping the two wavelengths equal to each other;
obtaining spectrum data from detection results obtained with the photodetector for each of the set wavelengths of the excitation-side light-dispersing system and the emission-side light-dispersing system; and
detecting an excitation wavelength of a sample from the obtained spectrum data.