Nanocarrier based plant transfection and transduction
AssigneeAgriculture and Agri-Food Canada
InventorsFrancois EUDES, Archana CHUGH
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Abstract
The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
Claims9
A method for delivering a protein to a plant, said method comprising exposing plant cells having a cell wall to a carrier-cargo complex comprising at least one polypeptide cargo moiety linked to a nuclear targeting cell penetrating peptide (CPP) carrier moiety and propagating the plant cells to form a plant, wherein the plant cells are embryo cells pre-treated with a cell permeabilizing agent comprising toluene or microspores.
The method according to claim 2, wherein the microspore is uninucleated or has an open micropore or is both uninucleated and has an open micropore.
Dependencies are inferred from the claim text.