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US 2014/0179940 A1Published application
Methods and compositions for improved production of fatty acids and derivatives thereof
AssigneeGenomatica, Inc.
InventorsDerek L. Greenfield, Louis G. Hom, Fernando A. Sanchez-Riera, Zhihao Hu, Vikranth Arlagadda, Eli S. Groban, Scott A. Frykman
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Abstract
The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.
Claims46
A method of producing a fatty acid or derivative thereof, comprising:
- (a) providing a host cell which is genetically engineered to have an altered level of expression of a FadR polypeptide as compared to the level of expression of the FadR polypeptide in a corresponding wild-type host cell,
- (b) culturing the engineered host cell in a culture medium under conditions permissive for the production of a fatty acid or derivative thereof, and
- (c) isolating the fatty acid or derivative thereof from the engineered host cell, wherein one or more of the titer, yield, or productivity of the fatty acid or derivative thereof produced by the engineered host cell is increased relative to that of the corresponding wild-type host cell.
Dependencies are inferred from the claim text.