Microfluidic device for nucleic acid extraction and fractionation
AssigneeQuantumdx Group Limited
InventorsJonathan James O'Halloran, Elaine Harrington Warburton, Matthew Daniel Solomon, John Edward McCormack, Matthias Schuenemann, David James Briggs, Mindy Lee Andre
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Abstract
Various embodiments of the present disclosure generally relate to molecular biological protocols, equipment and reagents for the extraction and fractionation of DNA molecules, from whole or lysed samples, in a single flow-through device.
Claims12
A device for simultaneously extracting and fractionating DNA from a lysate or a whole sample, the device comprising a single flow-through microfluidic channel, the channel comprising buffer and reagent chambers and a sorbent filter.
A method of fabricating a microfluidics device to extract and fractionate DNA from a sample, comprising:
- forming at least two blank layers having a channel;
- forming an adhesive layer with ports cut to through the layer corresponding to an inlet and an outlet port of the channel;
- vacuum packing a portion of the channel with a sorbent material;
- and
- aligning the blank layers with the adhesive layer therebetween and bonding the layers together under pressure.
Dependencies are inferred from the claim text.