US 2014/0179908 A1 · Chromatography column and method for isolating nucleic acid

Abstract

The present invention provides a chromatography column and a method for isolating nucleic acid molecules. In one embodiment, the present invention provides a double-layer column of a first anion exchange membrane and a second serially coupled silica membrane. Upon flowing a nucleic acid-containing solution through the first anion exchange membrane, the nucleic acid binds to and then elutes from the first membrane. The eluted solution then flows serially through the second silica membrane, which the nucleic acid binds to and then elutes from. Due to this novel serial coupled double-layer principle, the present invention is particularly suitable for co-isolating RNA and DNA, for isolating nucleic acid embraced by proteins, e.g., viruses, and for isolating diluted nucleic acid in a large volume, e.g., plasma. In addition, the eluted nucleic acid is ready for downstream applications.

Claims22

Dependent claims

A method for isolating nucleic acid, comprising:

a) providing a chromatography column comprising a first solid layer and a second solid layer which are serially coupled so that solutions flow through the first layer and then the second layer,
b) flowing a first aqueous solution containing nucleic acid through the first layer to which the nucleic acid becomes bound,
c) flowing a second aqueous solution through the first layer and eluting the bound nucleic acid, and then flowing the eluted solution through the second layer to which the eluted nucleic acid becomes bound, and
d) flowing a third aqueous solution through the second layer and eluting the bound nucleic acid.

1 more element

Dependencies are inferred from the claim text.

Chromatography column and method for isolating nucleic acid

Life of this patent

Chromatography column and method for isolating nucleic acid

Life of this patent

Abstract

Claims22