Neurogenesis screening method and uses thereof
OwnerChenzhong Kuang, Yan Xiao +3inventors
InventorsChenzhong Kuang, Yan Xiao, Dirk Hondmann, Eduard Poels, Zeina Jouni
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Abstract
Provided herein are methods for detecting and/or confirming the neurogenesis effect of eicosapentaenoic acid utilizing adipose-derived stem cells (ADSCs). Further provided are methods of promoting neurogenesis in ADSCs. Also provided are methods for the use of eicosapentaenoic acid for the production of a neurologic component that may be incorporated into a nutritional composition for promoting neurogenesis in a pediatric subject.
Claims20
A method for detecting neurogenesis of eicosapentaenoic acid, comprising:
- a) culturing adipose-derived stem cells (ADSCs) in the presence of eicosapentaenoic acid;
- and determining the extent of neurogenesis in the ADSCs cultured in the presence of eicosapentaenoic acid;
- b) culturing ADSCs in the absence of eicosapentaenoic acid and determining the extent of neurogenesis in the ADSCs cultured in the absence of eicosapentaenoic acid;
- and
- c) comparing the extent of neurogenesis in the ADSCs cultured in the presence of eicosapentaenoic acid to the extent of neurogenesis of the ADSCs cultured in the absence of eicosapentaenoic acid, and
- d) determining the extent of neurogenesis by observing a change in cell morphology of the ADSCs,
- wherein an increase in the extent of neurogenesis in the ADSCs cultured in the presence of eicosapentaenoic acid compared to the extent of neurogenesis in the ADSCs cultured in the absence of eicosapentaenoic acid indicates that the candidate compound is a neurogenesis-promoting compound.
A method for the use of eicosapentaenoic acid or combinations thereof for the production of a neurologic compound for promoting neurogenesis in a pediatric subject, comprising:
- providing a nutritional composition comprising a carbohydrate source, a fat source, a protein source, docosapentaenoic acid, linolenic acid, linoleic acid, and a neurologic component comprising eicosapentaenoic acid to a pediatric subject.
A method for the use of a neurologic component comprising eicosapentaenoic acid for promoting neurogenesis in a pediatric subject, comprising:
- providing a nutritional composition comprising per 100 kcal:
- from about 6 g to about 22 g of a carbohydrate source,
- from about 1.3 g to about 7.2 g of a fat source,
- from about 1.8 g to about 6.8 g of a protein source, and
- from about 5 g to about 100 g of a neurologic component comprising eicosapentaenoic acid.
Dependencies are inferred from the claim text.