Eicosapentaenoic acid (epa) formulations
AssigneeQualitas Health, Ltd.
InventorsBrian J. WAIBEL, Hans Schonemann, Val Krukonis, Michael Kagan
Life of this patent
Estimating the term…
Abstract
Provided herein are compositions comprising eicosapentaenoic acid (EPA) and polar lipids (e.g., glycolipids and phospholipids), and which do not contain any docosahexaenoic acid (DHA) or esterified fatty acids.
Claims42
An EPA composition comprising from about 15 wt. % to about 90 wt. % eicosapentaenoic acid (EPA) and about 10 wt. % to about 70 wt. % polar lipids, wherein the composition does not comprise docosahexaenoic acid (DHA) or esterified EPA, and wherein the composition is suitable for human consumption.
An EPA composition comprising following distribution of EPA by lipid class:
- about 3 wt. % to about 50 wt. % of the EPA is a phospholipid conjugate;
- about 5 wt. % to about 50 wt. % of the EPA is a glycolipid conjugate;
- about 0 wt. % to about 10 wt. % of the EPA is a triglyceride conjugate or a diglyceride conjugate;
- and
- about 15 wt. % to about 85 wt. % of the EPA is in free fatty acid form, wherein the composition is suitable for human consumption.
38-40. (canceled)
A method of producing a composition comprising EPA comprising:
- a) providing an algal paste;
- b) extracting lipids from the algal paste with an organic solvent, thereby substantially isolating a crude algae extract (CAE) comprising neutral lipids and polar lipids from the water-soluble components of the paste;
- c) substantially removing the remaining water-soluble components from the CAE, thereby yielding a crude algae oil (CAO);
- d) contacting the CAO with supercritical CO2, wherein the supercritical CO2 selectively extracts the neutral lipids, thereby splitting the CAO into a neutral lipid fraction comprising free fatty acids and a polar lipid fraction comprising glycolipids and phospholipids;
- e) isolating C20 free fatty acids from the neutral lipid fraction, thereby yielding a concentrated EPA free fatty acid fraction;
- and
- f) combining the concentrated EPA free fatty acid fraction produced in step e) and the polar lipid fraction produced in step d).
A method of producing a composition comprising EPA comprising:
- a) providing an algal paste;
- b) extracting lipids from the algal paste with an organic solvent, thereby isolating a crude algae extract (CAE) comprising neutral lipids and polar lipids from the water-soluble components of the paste;
- c) substantially removing the remaining water-soluble components from the CAE, thereby yielding a crude algae oil (CAO);
- d) hydrolyzing a first portion of the CAO, thereby releasing free fatty acids in the portion of CAO;
- e) fractionating the released free fatty acids according to chain length, thereby isolating C20 free fatty acids and yielding a concentrated EPA free fatty acid fraction;
- and
- f) combining the concentrated EPA free fatty acid fraction produced in step e) and a second portion of the CAO produced in step c).
43-64. (canceled)
Dependencies are inferred from the claim text.