Neurogenesis screening method and uses thereof
OwnerChenzhong Kuang, Yan Xiao +3inventors
InventorsChenzhong Kuang, Yan Xiao, Dirk Hondmann, Eduard Poels, Zeina Jouni
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Abstract
Provided herein are methods for detecting and/or confirming the neurogenesis effect of EGCG and/or resveratrol utilizing adipose-derived stem cells (ADSCs). Further provided are methods of promoting neurogenesis in ADSCs. Also provided are methods for the use of EGCG and/or resveratrol for the production of a neurologic component that may be incorporated into a nutritional composition for promoting neurogenesis in a pediatric subject.
Claims20
A method for detecting neurogenesis of epigallocatechin-gallate, resveratrol, or combinations thereof, comprising:
- a) culturing adipose-derived stem cells (ADSCs) in the presence of epigallocatechin-gallate or resveratrol;
- and determining the extent of neurogenesis in the ADSCs cultured in the presence of epigallocatechin-gallate or resveratrol;
- b) culturing ADSCs in the absence of epigallocatechin-gallate or resveratrol and determining the extent of neurogenesis in the ADSCs cultured in the absence of epigallocatechin-gallate or resveratrol;
- and
- c) comparing the extent of neurogenesis in the ADSCs cultured in the presence of epigallocatechin-gallate or resveratrol to the extent of neurogenesis of the ADSCs cultured in the absence of epigallocatechin-gallate or resveratrol, and
- d) determining the extent of neurogenesis by observing a change in cell morphology of the ADSCs,
- wherein an increase in the extent of neurogenesis in the ADSCs cultured in the presence of epigallocatechin-gallate or resveratrol compared to the extent of neurogenesis in the ADSCs cultured in the absence of epigallocatechin-gallate or resveratrol indicates that the candidate compound is a neurogenesis-promoting compound.
A method for the use of epigallocatechin-gallate, resveratrol or combinations thereof for the production of a neurologic compound for promoting neurogenesis in a pediatric subject, comprising:
- providing a nutritional composition comprising a carbohydrate source, a fat source, a protein source, and a neurologic component comprising epigallocatechin-gallate, resveratrol, or combinations thereof to a pediatric subject.
A method for the use of a neurologic component comprising epigallocatechin-gallate, resveratrol or combinations thereof for promoting neurogenesis in a pediatric subject, comprising:
- providing a nutritional composition comprising per 100 kcal:
- from about 6 g to about 22 g of a carbohydrate source,
- from about 1.3 g to about 7.2 g of a fat source,
- from about 1.8 g to about 6.8 g of a protein source,
- from about 0.01 g to about 18 mg of a neurologic component comprising epigallocatechin-gallate, resveratrol, or combinations thereof.
Dependencies are inferred from the claim text.