METHODS AND COMPOSITIONS REDUCING TARGET GENE EXPRESSION USING COCKTAILS OF siRNAS OR CONSTRUCTS EXPRESSING siRNAS
AssigneeApplied Biosystems, LLC
InventorsDAVID BROWN, LANCE FORD, RICHARD JARVIS
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Abstract
The present invention concerns methods and compositions involving the production or generation of siRNA mixtures or pools capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include kits that include reagents for producing or generating siRNA pools. The present invention further concerns methods using polypeptides with RNase III activity for generating siRNA mixtures or pools that effect RNAi, including the generation of a number of RNA molecules to the same target gene.
Claims12
A method for reducing expression of a target gene in a cell comprising introducing a pool of siRNA molecules directed to the target gene into the cell, wherein the pool consists of four different siRNA molecules, and wherein the target gene is Rho, CDC2 or Survivin.
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A composition for reducing expression of a target gene in a cell comprising a pool of siRNAs directed to the target gene, wherein the pool consists of four different siRNA molecules, and wherein the target gene is Rho, CDC2 or Survivin.
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Dependencies are inferred from the claim text.