Methods for Treating or Preventing Ophthalmological Diseases

A method for treating wet age-related macular degeneration, comprising administering to a human in need thereof an effective amount of:

• (a) a pegylated anti-PDGF-B aptamer having the formula 5′-[mPEG2 40 kD]-[HN—(CH2)6O] CAGGCUfACfGm [PO3(CH2CH2O)6] CGTAGmAGmCAUfCfAm [PO3(CH2CH2O)6] TGATCfCfUfGm-[3T]-3′ (SEQ ID NO:
• 23),
• where
• [3T] refers to an inverted thymidine nucleotide that is attached to the 3′ end of the oligonucleotide at the 3′ position on the ribose sugar;
• [mPEG2 40 kD] represents two about 20 kD polyethylene glycol (PEG) polymer chains that are covalently attached to the two amino groups of a lysine residue via carbamate linkages;
• the [mPEG2 40 kD]-lysine moiety is covalently attached to the [HN—(CH2)6—O] linker via an amide bond;
• Gm and Am represent 2′-methoxy substituted forms of guanosine and adenosine, respectively;
• and
• Cf and Uf represent 2′-fluoro substituted forms of cytosine and uridine, respectively,
• or a pharmaceutically acceptable salt thereof;
• and
• (b) aflibercept, or a pharmaceutically acceptable salt thereof.