Streptococcus bacteriophage lysins for treatment of gram positive bacteria in companion animals and livestock
AssigneesThe Rockefeller University, Universiteit Utrecht Holding B.V.
InventorsVincent A. Fischetti, Jonathan Schmitz, Daniel Gilmer, Jacob Anthony Wagenaar, Jan Arend Stegeman
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Abstract
The present invention provides methods for the prophylactic and therapeutic amelioration and treatment of gram-positive bacteria in companion animals and livestock, including Streptococcus and Staphylococcus bacterial strains, including pathogenic and antibiotic-resistant bacteria, and related conditions. The methods of the invention utilize PlySs2 and/or PlySs1 lytic enzymes and variants thereof, including truncations thereof. Methods for treatment of companion animals and livestock are provided.
Claims15
A method for treating gram-positive bacterial infection caused by one or more of Staphylococcus, Streptococcus, Enterococcus or Listeria bacteria in a companion animal or livestock comprising the step of administering to the companion animal or livestock having a bacterial infection, an effective amount of a composition comprising an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:3 or variants thereof having at least 80% homology to the polypeptide of SEQ ID NO:3 and effective to kill the gram-positive bacteria, whereby the number of gram-positive bacteria in the companion animal or livestock is reduced and the infection is controlled.
(canceled)
A method for treating gram-positive bacterial infection caused by one or more of Staphylococcus, Streptococcus, Enterococcus or Listeria bacteria in a companion animal or livestock comprising the step of administering to the companion animal or livestock having a bacterial infection, an effective amount of a composition comprising an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:1, the isolated lysin polypeptide comprising the amino acid sequence of SEQ ID NO:2, or variants thereof having at least 80% homology to the polypeptide of SEQ ID NO:1 or of SEQ ID NO:2 and effective to kill the gram-positive bacteria, whereby the number of gram-positive bacteria in the companion animal or livestock is reduced and the infection is controlled.
A method for treating a companion animal or livestock subject exposed to or at risk for exposure to a pathogenic gram-positive bacteria comprising the step of administering to the companion animal or livestock subject a composition comprising an isolated lysin polypeptide comprising the amino acid sequence of SEQ ID NO:1, the isolated lysin polypeptide comprising the amino acid sequence of SEQ ID NO:2, or variants thereof having at least 80% homology to the polypeptide of SEQ ID NO:1 or of SEQ ID NO:2 and effective to kill the gram-positive bacteria
A method for reducing or controlling gram-positive bacterial infection or contamination caused by one or more of Staphylococcus, Streptococcus, Enterococcus or Listeria bacteria in a companion animal or livestock comprising the step of contacting the exterior surface, skin, or coat of the companion animal or livestock with an effective amount of a composition comprising an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:1, the isolated lysin polypeptide comprising the amino acid sequence of SEQ ID NO:2, or variants thereof having at least 80% homology to the polypeptide of SEQ ID NO:1 or of SEQ ID NO:2 and effective to kill the gram-positive bacteria, whereby the number of gram-positive bacteria in the companion animal or livestock is reduced and the infection is controlled.
(canceled)
(canceled)
Dependencies are inferred from the claim text.