Compositions and methods for selection of nucleic acids containing modified bases
AssigneePacific Biosciences of California, Inc.
InventorsJonas Korlach, Tyson A. Clark, Stephen Turner
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Abstract
Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis. The methods result in a subset of the initial population enriched for a desired property, or lacking nucleic acids having an undesired property. The methods are particularly useful for analyzing populations having a high degree of complexity, e.g., chromosomal-derived DNA, whole genomic DNA, or mRNA populations. In addition, such methods allow for analysis of pooled samples.
Claims20
A method of generating a pool of sequencing templates enriched for a type of modified base, the method comprising:
- a) fragmenting a nucleic acid sample comprising the type of modified base, thereby generating a mixture comprising a first subset of nucleic acid fragments comprising the type of modified base and a second subset of nucleic acid fragments that do not comprise the type of modified base;
- b) linking adapters to nucleic acid fragments in both the first subset and the second subset;
- and
- c) retaining the nucleic acid fragments in the first subset from the mixture, thereby generating a pool of sequencing templates enriched for the type of modified base.
Dependencies are inferred from the claim text.