Method and Nucleic Acids for the Analysis of Colorectal Cell Proliferative Disorders
AssigneeEpigenomics AG
InventorsPeter Adorjan, Matthias Burger, Sabine Maier, Ralf Lesche, Susan Cottrell, Theo De Vos
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Abstract
The invention provides methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders as well as therapy thereof by analysis of the gene EYA4 and its promoter and regulatory sequences. The invention further provides novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said gene as well as methods, assays and kits thereof.
Claims14
A nucleic acid comprising a sequence at least 18 bases in length of a segment of the chemically pretreated genomic DNA according to one of the sequences taken from the group comprising SEQ ID NO:
- 2 to SEQ ID NO:
- 5 and sequences complementary thereto.
An oligomer, in particular an oligonucleotide or peptide nucleic acid (PNA)-oligomer, said oligomer comprising in each case at least one base sequence having a length of at least 9 nucleotides which is complementary to, or hybridizes under moderately stringent or stringent conditions to a pretreated genomic DNA according to one of the SEQ ID NO:
- 2 to SEQ ID NO:
- 5 and sequences complementary thereto.
A composition of matter comprising the following:
- a nucleic acid comprising a sequence at least 18 bases in length of a segment of the chemically pretreated genomic DNA according to one of the sequences taken from the group comprising SEQ ID NO:
- 1 to SEQ ID NO:
- 5 and sequences complementary thereto, and
- a buffer comprising at least one of the following substances:
- 1 to 5 mM Magnesium Chloride, 100-500 μM dNTP, 0.5-5 units of taq polymerase, an oligomer, in particular an oligonucleotide or peptide nucleic acid (PNA)-oligomer, said oligomer comprising in each case at least one base sequence having a length of at least 9 nucleotides which is complementary to, or hybridizes under moderately stringent or stringent conditions to a pretreated genomic DNA according to one of the SEQ ID NO:
- 2 to SEQ ID NO:
- 5 and sequences complementary thereto.
A kit useful for detecting, differentiating or distinguishing between colon cell proliferative disorders, comprising:
- a) a bisulfite reagent;
- and
- b) at least one nucleic acid molecule or peptide nucleic acid molecule comprising, in each case a contiguous sequence at least 9 nucleotides in length that is complementary to, or hybridizes under moderately stringent or stringent conditions to a sequence selected from the group consisting of SEQ ID NOS:
- 1 to 5, and complements thereof.
Dependencies are inferred from the claim text.