US 2014/0179541 A1 · MODIFYING SINGLE PROTEINS (GPCR), LIGANDS, AND NANOPORE SURFACES TO CREATE BINDING-INDUCED MOLECULAR CHANGES OF PROTEIN-LIGAND COMPLEXES DETECTED IN NANOCHANNEL TRANSLOCATION

Abstract

A mechanism is provided for utilizing a nanodevice to distinguish molecules with different structure. The molecules translocate through or across a nanochannel filled with a electrolyte solution. An electrical signal through the nanochannel is measured for every translocation event. Inner surfaces of the nanochannel include a functional layer, which is a coating to functionalize the nanochannel, in which the functional layer is configured to interact with predetermined ones of the molecules during translocation events. It is determined that a combination of at least two different molecules is formed based on predetermined ones of the molecules interacting with the functional layer to change the electrical signal and/or change a translocation time for the translocation event.

Claims18

Dependent claims

A method for determining an association of molecules with different structures, the method comprising:

translocating the molecules partially or entirely through one or more nanochannels filled with an electrolyte solution based on an electric potential difference applied in a longitudinal direction of the one or more nanochannels;
measuring one or more electrical signals through, across, or both through and across the one or more nanochannels for every event, the event being a translocation event, a binding event, or both the translocation event and the binding event;
and
determining that at least two different molecules have combined into a combination based on a change in the one or more electrical signals, a change in translocation time for the translocation event, or both a change in the one or more electrical signals and in the translocation time for the translocation event;
wherein the molecules are chosen from a group comprising proteins, electrically charged molecules, electrically neutral molecules, organic compounds, inorganic compounds, and combinations thereof;
and
wherein the proteins are G-protein coupled receptors.

4 more elements

Dependencies are inferred from the claim text.

Modifying single proteins (gpcr), ligands, and nanopore surfaces to create binding-induced molecular changes of protein-ligand complexes detected in nanochannel translocation

Life of this patent

Modifying single proteins (gpcr), ligands, and nanopore surfaces to create binding-induced molecular changes of protein-ligand complexes detected in nanochannel translocation

Life of this patent

Abstract

Claims18