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AssigneePrecision BioSciences, Inc.
InventorsDerek JANTZ, James Jefferson SMITH, Michael G. NICHOLSON
Estimating the term…
Methods of inserting genes into defined locations in the chromosomal DNA of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest. The invention also relates to meganucleases, vectors and engineered cell lines necessary for performing the methods, to cell lines resulting from the application of the methods, and use of the cell lines to produce protein products of interest.
1-19. (canceled)
A method for inserting an exogenous sequence into an amplifiable locus of a mammalian cell comprising:
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A recombinant meganuclease comprising a polypeptide having at least 75%, 85%, 90%, 95%, 97%, 98% or 99% sequence identity to SEQ ID NO:
The recombinant meganuclease of 55, having the sequence of the meganuclease of SEQ ID NO:
A recombinant meganuclease which recognizes and cleaves a recognition site having at least 75%, 85%, 90%, 95%, 97%, 98% or 99% sequence identity to SEQ ID NO:
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Dependencies are inferred from the claim text.