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US 2014/0179004 A1Published application
Cell for use in immunotherapy which contains modified nucleic acid construct encoding wilms tumor gene product or fragment thereof, method for producing said cell, and said nucleic acid construct
AssigneeRiken
InventorsShin-ichiro Fujii, Kanako Shimizu, Jun Shinga
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Abstract
A cell of the present invention contains a nucleic acid construct encoding a WT1 gene product or a fragment of the WT1 gene product. The nucleic acid construct contains (i) a region encoding a desired fragment of the WT1 gene product and (ii) only AUG as a functional start codon. The present invention can provide a cell into which the nucleic acid construct is introduced so that an expression level of a WT1 gene product or a fragment of the WT1 gene product is remarkably enhanced.
Claims10
A cell for immunotherapy, comprising:
- a nucleic acid construct encoding a Wilms tumor gene product or a fragment of the Wilms tumor gene product,
- the nucleic acid construct including (i) a region encoding a fragment of the Wilms tumor gene product, the fragment being indicated by positions 194 to 493 of SEQ ID NO:
- 1 or by positions corresponding to the positions 194 to 493 of a sequence corresponding to SEQ ID NO:
- 1 and (ii) only one AUG as a functional start codon, connected to a 5′ terminal side of the region via 3m (m is 0 or a positive integer) bases intervening between the 5′ terminal side of the region and the AUG as the functional start codon.
1 more element
Dependencies are inferred from the claim text.