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US 2014/0179003 A1Published application
Alternative export pathways for vector expressed rna interference
AssigneeUniversity of Iowa Research Foundation
InventorsScott Harper, Beverly L. Davidson
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Abstract
The present invention is directed to nucleic acid molecules containing a loop sequence designed to circumvent exportin-5 mediated export, and methods using these novel molecules.
Claims20
An isolated nucleic acid molecule comprising
- a first portion, wherein the first portion is no more than 30 nucleotides in length;
- a second portion, wherein the second portion has a sequence that is complementary to the first portion;
- and
- a loop portion comprising a sequence designed to circumvent exportin-5 mediated export;
- wherein the first portion and the second portion are operably linked by means of the loop portion to form a hairpin structure comprising a duplex structure and a loop structure.
2 more elements
An expression cassette comprising a sequence encoding a nucleic acid molecule comprising:
- a promoter;
- a first portion, wherein the first portion is no more than 30 nucleotides in length;
- a second portion, wherein the second portion has a sequence that is complementary to the first portion;
- and
- a loop portion comprising a sequence designed to circumvent exportin-5 mediated export;
- wherein the first portion and the second portion are operably linked by means of the loop portion to form a hairpin structure comprising a duplex structure and a loop structure.
3 more elements
Dependencies are inferred from the claim text.