Culture control method, cell culture apparatus, and apparatus for evaluation of cellular characteristics
AssigneeHitachi, Ltd.
InventorsKeisuke SHIBUYA, Ryoichi HAGA, Masaru NAMBA, Kenichiro OKA, Ken AMANO
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Abstract
This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.
Claims10
A culture control method comprising performing cell culture under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.
A cell culture apparatus comprising:
- a culture vessel comprising of an agitation blade and a drive unit that allows the agitation blade to revolve;
- and
- a control unit that controls the drive unit to realize agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.
An apparatus for evaluation of cellular characteristics comprising:
- a support onto which cells are fixed via anchor molecules;
- a chamber comprising an inlet port at one end thereof and an outlet port at another end thereof, in which the support is mounted between the inlet port and the outlet port;
- a culture solution feeder that supplies a culture solution at a desired flow rate from the inlet port toward the outlet port;
- and
- an analyzer that analyzes components contained in the culture solution discharged from the outlet port.
Dependencies are inferred from the claim text.