Membrane-separation-type culture device, membrane-separation-type culture kit, stem cell separation method using same, and separation membrane
AssigneeNational Center for Geriatrics and Gerontology
InventorsMisako Nakashima, Koichiro Iohara, Kazumasa Yamada, Masaaki Shimagaki, Masahiro Osabe
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Abstract
A membrane separation culture device includes an upper structure including a vessel in which at least a portion of the bottom thereof is formed with a separation membrane having pores that allow stem cells to permeate therethrough, and a lower structure including a vessel that retains a fluid in which the membrane of the upper structure is immersed.
Claims32
A membrane separation culture device comprising:
- an upper structure comprising a vessel in which at least a portion of the bottom thereof is formed with a separation membrane having pores that allow stem cells to permeate therethrough;
- and
- a lower structure comprising a vessel that retains a fluid in which the membrane of the upper structure is immersed.
A separation membrane comprising:
- a base material membrane consisting of a hydrophobic polymer;
- and
- a functional layer formed by allowing one or more hydrophilic polymers selected from a vinyl pyrrolidone polymer, a polyethylene glycol polymer and a vinyl alcohol polymer to bind to the surface of the base material membrane via a covalent bond;
- wherein
- weight percentage of the hydrophilic polymer(s) constituting the functional layer is 1.5% to 35% based on the total weight of the separation membrane.
A method of modifying a surface of a molded body, comprising:
- immersing a molded body having a water absorption percentage of 2% or less in a treating aqueous solution containing one or more hydrophilic polymers selected from a vinyl pyrrolidone polymer, a polyethylene glycol polymer and a vinyl alcohol polymer at a concentration of 10 to 2000 ppm, and also containing a 0.01% to 0.2% alcohol;
- and
- irradiating the molded body with a high-energy beam to modify the surface of the molded body to have a protein adhesion-suppressing property and a cell adhesion-suppressing property.
A method of modifying a surface of a molded body comprising:
- of immersing a molded body having a water absorption percentage of more than 2% in an aqueous solution containing one or more hydrophilic polymers selected from a vinyl pyrrolidone polymer, a polyethylene glycol polymer and a vinyl alcohol polymer at a concentration of 10 to 2000 ppm;
- and
- irradiating the molded body with a high-energy beam to modify the surface of the molded body to have a protein adhesion-suppressing property and a cell adhesion-suppressing property.
Dependencies are inferred from the claim text.