Induced pluripotent stem cells from human umbilical cord tissue-derived cells
AssigneeDePuy Synthes Products, Inc.
InventorsCharito Buensuceso, Agineszka Seyda, David C. Colter, Sridevi Dhanaraj, Brian C. Kramer
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Abstract
We have disclosed an induced pluripotent stem cell and the method of preparing the induced pluripotent stem cell from a human umbilical cord tissue-derived cell. More particularly, we have disclosed a human umbilical cord tissue-derived iPS cell which may be differentiated into cells of ectoderm, mesoderm, and endoderm lineages.
Claims7
1-7. (canceled)
A human umbilical cord tissue-derived cell wherein the human umbilical cord tissue-derived cell is an isolated umbilical cord tissue cell isolated from human umbilical cord tissue substantially free of blood that is capable of self-renewal and expansion in culture, has the potential to differentiate into cells of other phenotypes, can undergo at least 40 doublings in culture, maintains a normal karyotype upon passaging, has the following characteristics:
- expresses each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, and HLA-A,B,C;
- does not express any of CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G, or HLA-DR,DP,DQ;
- and increased expression of a gene for each of interleukin 8;
- reticulon 1;
- and chemokine (C-X-C motif) ligand 3 relative to that of a human cell which is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell, and is transfected with murine retroviruses, individually carrying constitutively expressed human transcription factors OCT4, SOX2, KLF4, and c-MYC.
The human umbilical cord tissue-derived cell of claim 1 wherein the murine retrovirus further carries p53-shRNA.
The human umbilical cord tissue-derived cell of claim 1 wherein the human umbilical cord tissue-derived cell further has the following characteristics:
- secretes each of the factors MCP-1, MIP1beta, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, RANTES and TIMP1;
- and
- does not secrete any of the factors SDF-1alpha, TGF-beta2, ANG2, PDGFbb, MIP1a and VEGF.
The human umbilical cord tissue-derived cell of claim 1 wherein the human umbilical cord tissue-derived cell expresses TRA1-60, TRA1-81, SSEA3, SSEA4, and NANOG.
The human umbilical cord tissue-derived cell of claim 1 wherein the human umbilical cord tissue-derived cell is positive for alkaline phosphatase staining.
The human umbilical cord tissue-derived cell of claim 1 wherein the human umbilical cord tissue-derived cell differentiates into cells of ectoderm, mesoderm, and endoderm lineages.
Dependencies are inferred from the claim text.