Method for culturing mammalian cells to improve recombinant protein production
AssigneeAmgen Inc.
InventorsMatthew I. Jerums, Amanda Kano, Henry Lin, Shun Luo, Jian Wu, Rebecca E. McCoy, Arvia E. Morris
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Abstract
The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.
Claims19
A method of culturing Chinese Hamster Ovary (CHO) cells expressing a recombinant protein, comprising growing the CHO cells in a defined serum-free culture medium during a growth phase and maintaining the CHO cells in the cell culture medium during a production phase by supplementing the cell culture with a concentrated defined serum-free feed medium that does not contain tyrosine, cysteine or cystine, and further supplementing the cell culture with an independent tyrosine and cystine feed, wherein viability was prolonged, specific productivity and titer was not diminished by the independent tyrosine and cystine feed.
A method of culturing Chinese Hamster Ovary (CHO) cells expressing a recombinant protein, comprising growing the CHO cells in a defined serum-free culture medium during a growth phase and maintaining the CHO cells in the cell culture medium during a production phase by supplementing the cell culture with a concentrated serum-free defined feed medium containing tyrosine and further supplementing the cell culture with an independent tyrosine feed, wherein viability was prolonged, specific productivity was maintained, and titer was improved compared to CHO cells not receiving independent tyrosine feeds.
Dependencies are inferred from the claim text.