Cyanobacterium sp. for Production of Compounds
AssigneeAlgenol Biofuels Inc.
InventorsIrina Piven, Alexandra Friedrich, Ulf Dühring, Frank Uliczka, Kerstin Baier, Masami Inaba, Tuo Shi, Kui Wang, Heike Enke, Dan Kramer
Life of this patent
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Abstract
A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.
Claims30
A non-naturally occurring ethanologenic Cyanobacterium sp. organism derived from Cyanobacterium sp. ABICyano1 wherein said organism comprises a genetically enhanced plasmid derived from a plasmid that is endogenous to Cyanobacterium sp. ABICyano1, and wherein said organism produces ethanol from about 0.002% (vol/vol) per day up to about 0.07% (vol/vol) per day.
A non-naturally occurring ethanologenic Cyanobacterium sp. organism comprising Cyanobacterium sp. ABICyano1 deposited in the American Type Culture Collection (ATCC) under ATCC accession number PTA-13311, and
- further comprising an ethanologenic pathway comprising one, two, or three exogenous nucleic acids each encoding an ethanologenic pathway enzyme expressed in a sufficient amount to produce ethanol, and
- wherein said ethanologenic pathway has been introduced into the chromosomal DNA of said Cyanobacterium sp. ABICyano1, and
- wherein said ethanologenic pathway comprises at least one pyruvate decarboxylase gene operably linked to a promoter and an alcohol dehydrogenase gene operably linked to a promoter.
A method for producing ethanol comprising growing a non-naturally occurring ethanologenic Cyanobacterium sp. organism comprising Cyanobacterium sp. ABICyano1 deposited in the American Type Culture Collection (ATCC) under ATCC accession number PTA-13311, and further comprising an ethanologenic pathway comprising at least one exogenous nucleic acid encoding an ethanologenic pathway enzyme expressed in a sufficient amount for the organism to produce ethanol, said ethanologenic pathway comprising a pyruvate decarboxylase, and an alcohol dehydrogenase.
Dependencies are inferred from the claim text.