Method for amplifying the pentose phosphate pathway in yeast strains, the resulting yeast and uses thereof
OwnerSylvie Dequin, Anne Ortiz-Julien +1inventors
InventorsSylvie Dequin, Anne Ortiz-Julien, Axelle Cadiere
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Abstract
The invention relates to a method for obtaining mutant yeast strains having an amplified PPP, characterised in that it comprises the culture of the strains on a substrate containing a gluconic acid salt.
Claims9
A method for obtaining mutant yeast strains having an amplified PPP, characterized in that it comprises culturing the strains on a substrate based on a gluconic acid salt.
Yeast strain mutants which have an amplified PPP compared with a parental strain, characterized in that they are capable of being obtained by means of a method of culture comprising the steps
- of adapting a yeast strain, over at least 10 generations, using as sole carbon source a substrate based on gluconate,
- of selecting the mutants having accumulated mutations allowing amplification of the PPP, having a final OD at least 1.3 times greater than that of the parental strain not subjected to the above adaptation conditions.
The mutant strain of Saccharomyces cerevisiae deposited at the Collection Nationale de Culture de Microorganismes (CNCM) [French National Microorganism Culture Collection], 25 rue du Dr Roux, Paris, for the purposes of a filing according to the Treaty of Budapest, under No. CNCM I-4216, on Jul. 29, 2009.
Dependencies are inferred from the claim text.