New means and methods for producing propanediol
AssigneeB.R.A.I.N. Biotechnology Research and Information Network AG
InventorsDaniel Koch, Guido Meurer, Jürgen Eck
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Abstract
The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.
Claims19
A host cell having an elevated expression or activity of at least two enzymes as compared with the parent cell from which it has been derived, said enzymes being
- (a) an enzyme having lactoyl-CoA reductase activity;
- and
- (b) (i) lactate-CoA transferase, or
- (ii) lactaldehyde reductase.
A host cell engineered with one or more nucleic acids encoding
- (a) an enzyme having lactoyl CoA-reductase activity;
- and
- (b) (i) lactate-CoA transferase, or
- (ii) lactaldehyde reductase.
A kit comprising
- (a) an enzyme with lactoyl-CoA reductase activity;
- and
- (b) (i) lactate CoA-transferase;
- or
- (ii) lactaldehyde reductase;
- or one or more nucleic acids encoding said enzymes according to (a) and (b).
Use of an enzyme selected from succinyl-CoA reductases, malonyl-CoA reductases, butyryl-CoA reductases, propionyl-CoA reductases and acetyl-CoA reductases for providing lactoyl-CoA reductase activity.
Dependencies are inferred from the claim text.