Adipate (ester or thioester) synthesis

A non-naturally occurring microbial organism, comprising a microbial organism having an adipate thioester pathway comprising at least one exogenous nucleic acid encoding a set of adipate thioester pathway enzymes expressed in a sufficient amount to produce an adipate thioester, wherein said set of adipate thioester pathway enzymes convert a succinate thioester and an acetate thioester to a 3-oxoadipate thioester, a 3-oxoadipate thioester to a 3-hydroxyadipate thioester, a 3-hydroxyadipate thioester to a 5-carboxy-2-pentenoate thioester and a 5-carboxy-2-pentenoate thioester to an adipate thioester.

A method for producing adipyl-CoA, comprising culturing a host microbial organism in which at least one exogenous nucleic acid is introduced under conditions and for a sufficient period of time to produce adipyl-CoA, wherein said host microbial organism comprises at least one exogenous nucleic acid encoding a set of adipyl-CoA pathway enzymes that are expressed in a sufficient amount to produce adipyl-CoA, wherein said set of adipyl-CoA pathway enzymes convert succinyl-CoA and acetyl-CoA to 3-oxoadipyl-CoA, 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, 3-hydroxyadipyl-CoA to 5-carboxy-2-pentenoyl-CoA and 5-carboxy-2-pentenoyl-CoA to adipyl-CoA.

A method for producing adipate, comprising culturing a host microbial organism in which at least one exogenous nucleic acid is introduced under conditions and for a sufficient period of time to produce adipate, wherein said host microbial organism comprises at least one exogenous nucleic acid encoding a set of adipyl-CoA pathway enzymes that are expressed in a sufficient amount to produce adipyl-CoA, wherein said set of adipyl-CoA pathway enzymes convert succinyl-CoA and acetyl-CoA to 3-oxoadipyl-CoA, 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, 3-hydroxyadipyl-CoA to 5-carboxy-2-pentenoyl-CoA and 5-carboxy-2-pentenoyl-CoA to adipyl-CoA, and wherein said host microbial organism comprises at least one exogenous nucleic acid encoding an adipate pathway enzyme expressed in a sufficient amount to produce adipate, wherein said adipate pathway enzyme is selected from the group consisting of an adipyl-CoA synthetase, a phosphotransadipylase/adipate kinase, an adipyl-CoA:acetyl-CoA transferase and an adipyl-CoA hydrolase.

A method for producing adipate, comprising culturing a host microbial organism in which at least one exogenous nucleic acid is introduced under conditions and for a sufficient period of time to produce adipate, wherein said host microbial organism comprises at least one exogenous nucleic acid encoding a set of adipyl-CoA pathway enzymes that are expressed in a sufficient amount to produce adipyl-CoA, wherein said set of adipyl-CoA pathway enzymes convert succinyl-CoA and acetyl-CoA to 3-oxoadipyl-CoA, 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, 3-hydroxyadipyl-CoA to 5-carboxy-2-pentenoyl-CoA and 5-carboxy-2-pentenoyl-CoA to adipyl-CoA, and wherein said host microbial organism comprises at least one exogenous nucleic acid encoding an adipate pathway enzyme expressed in a sufficient amount to produce adipate, wherein said adipate pathway enzyme is selected from the group consisting of a succinyl-CoA:acetyl-CoA acyl transferase, a 3-oxoadipyl-CoA transferase, a 3-oxoadipate reductase, a 3-hydroxyadipate dehydratase, and a 2-enoate reductase.

A recombinant microorganism comprising at least one exogenous nucleic acid encoding enzymes that catalyze reactions that result in the production of an adipate thioester, wherein said enzymes convert a succinate thioester and an acetate thioester to a 3-oxoadipate thioester, a 3-oxoadipate thioester to a 3-hydroxyadipate thioester, a 3-hydroxyadipate thioester to a 5-carboxy-2-pentenoate thioester and a 5-carboxy-2-pentenoate thioester to an adipate thioester.