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AssigneeEvonik Industries AG
InventorsSteffen SCHAFFER, Jasmin Gielen, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Thomas Haas, Wilfried Bluemke
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The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.
A whole cell catalyst which expresses a transaminase and one selected from the group consisting of an α-dioxygenase or a combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phospho-pantetheinylating the fatty acid reductase, wherein at least one of the phosphopantetheinyl transferase and transaminase is recombinant.
A method for preparing an amine, the method comprising:
Dependencies are inferred from the claim text.