Meganuclease variants cleaving at least one target in the genome of a retrovirus and uses thereof
AssigneeCellectis
InventorsANDRE CHOULIKA, ROMAN GALETTO
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Abstract
Meganuclease variants which cleave at least one target in the provirus of a retrovirus and in particular which cleave the genomic insertion of the provirus. The present invention particular relates to meganuclease variants which cleave the provirus of the Human Immunodeficiency Virus genome following genomic insertion. Vector encoding such variants, as well as to a cell or multi-cellular organism modified by such a vector and use of said meganuclease variants and derived products for genome engineering and for in vivo and ex vivo (gene cell therapy) genome therapy.
Claims16
A method of cleaving a DNA target sequence in the provirus of a pathogenic retrovirus comprising contacting said DNA target sequence with an I-CreI variant to thereby cleave said DNA target sequence
- wherein said I-CreI variant comprises a first monomer and a second monomer which are associated to form an active form,
- wherein said I-CreI variant comprises at least two substitutions in at least one of the monomers,
- wherein at least one substitution is of a residue in the range of positions 26 to 40 of I-CreI and at least one substitution is of a residue in the range of positions 44 to 77 of I-CreI
- wherein all position numbering herein corresponds to SEQ ID NO:
- 344 and
- wherein said DNA target sequence is at least one sequence selected from the group consisting of SEQ ID NO:
- 319 to 342 and SEQ ID NO:
- 366 to 368.
Dependencies are inferred from the claim text.