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US 2014/0178940 A1Published application
Compositions and methods for the protection of nucleophilic groups
AssigneeAlleLogic Biosciences Corporation
InventorsFei Mao, Xing Xin, Wai-Yee Leung
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Abstract
The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention.
Claims6
A method for preparing a protected lysine residue in a polypeptide comprising
- i) reacting a six-membered or seven-membered cyclic anhydride with an ε-amino group of a lysine residue, wherein the reaction results in a protected lysine residue comprising an amide and a carboxylic acid;
- and further wherein the carboxylic acid is conformationally constrained with respect to the amide such that the carboxylic acid exhibits a greater propensity to react with the amide relative to a corresponding protected lysine residue comprising an unconstrained carboxylic acid;
- or
- ii) reacting a five-membered cyclic anhydride with an ε-amino group of a lysine residue, wherein the reaction results in a protected lysine residue comprising an amide and a carboxylic acid;
- and further wherein the carboxylic acid is conformationally constrained with respect to the amide by a substituted 3, 4, 5, 6 or 7-membered ring such that the carboxylic acid exhibits a greater propensity to react with the amide relative to a corresponding protected lysine residue comprising an unconstrained carboxylic acid.
2 more elements
Dependencies are inferred from the claim text.