Hepatocyte growth factor (hgf) binding proteins
AssigneeAVEO Pharmaceuticals, Inc.
InventorsWilliam M. Winston, JR., S. Kirk Wright, May Han, Lyne Breault, Jie Lin, Bijan Etemad-Gilbertson, Christine Knuehl, Jeno Gyuris, Arnold Horwitz
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Abstract
The present invention provides a family of binding proteins that bind and neutralize the activity of hepatocyte growth factor (HGF), in particular human HGF. The binding proteins can be used as diagnostic and/or therapeutic agents. With regard to their therapeutic activity, the binding proteins can be used to treat certain HGF responsive disorders, for example, certain HGF responsive tumors.
Claims24
1-28. (canceled)
An isolated nucleic acid comprising a nucleotide sequence encoding an immunoglobulin heavy chain variable region comprising the structure CDRH1-CDRH2-CDRH3, wherein
- (i) CDRH1 comprises the amino acid sequence of SEQ ID NO:
- 15,
- (ii) CDRH2 comprises the amino acid sequence of SEQ ID NO:
- 204, and
- (iii) CDRH3 comprises the amino acid sequence of SEQ ID NO:
- 17;
- and
- an immunoglobulin light chain variable region comprising the structure CDRL1-CDRL2-CDRL3, wherein
- (i) CDRL1 comprises the amino acid sequence of SEQ ID NO:
- 18,
- (ii) CDRL2 comprises the amino acid sequence of SEQ ID NO:
- 19, and
- (iii) CDRL3 comprises the amino acid sequence of SEQ ID NO:
- 20;
- wherein the immunoglobulin heavy chain variable region and the immunoglobulin light chain variable region together bind human hepatocyte growth factor (HGF).
A host cell comprising a first expression vector comprising a nucleic acid sequence encoding an immunoglobulin heavy chain variable region comprising the structure CDRH1-CDRH2-CDRH3, wherein
- (i) CDRH1 comprises the amino acid sequence of SEQ ID NO:
- 15,
- (ii) CDRH2 comprises the amino acid sequence of SEQ ID NO:
- 204, and
- (iii) CDRH3 comprises the amino acid sequence of SEQ ID NO:
- 17;
- and
- a second expression vector comprising a nucleic acid sequence encoding an immunoglobulin light chain variable region comprising the structure CDRL1-CDRL2-CDRL3, wherein
- (i) CDRL1 comprises the amino acid sequence of SEQ ID NO:
- 18,
- (ii) CDRL2 comprises the amino acid sequence of SEQ ID NO:
- 19, and
- (iii) CDRL3 comprises the amino acid sequence of SEQ ID NO:
- 20;
- wherein the immunoglobulin heavy chain variable region and the immunoglobulin light chain variable region together bind human hepatocyte growth factor (HGF).
An isolated nucleic acid comprising a nucleotide sequence encoding an immunoglobulin heavy chain variable region comprising the amino acid sequence of SEQ ID NO:
- 183, and encoding an immunoglobulin light chain variable region comprising the amino acid sequence of SEQ ID NO:
- 193, wherein the immunoglobulin heavy chain variable region and the immunoglobulin light chain variable region together bind human hepatocyte growth factor (HGF).
A host cell comprising:
- a first expression vector comprising a nucleic acid sequence encoding an immunoglobulin heavy chain variable region comprising the amino acid sequence of SEQ ID NO:
- 183;
- and
- a second expression vector comprising a nucleic acid sequence encoding an immunoglobulin light chain variable region comprising amino acid sequence of SEQ ID NO:
- 193;
- wherein the immunoglobulin heavy chain variable region and the immunoglobulin light chain variable region together bind human hepatocyte growth factor (HGF).
An isolated nucleic acid comprising a nucleotide sequence encoding an immunoglobulin heavy chain comprising the amino acid sequence of SEQ ID NO:
- 187, and encoding an immunoglobulin light chain comprising the amino acid sequence of SEQ ID NO:
- 197, wherein the immunoglobulin heavy chain variable region and the immunoglobulin light chain variable region together bind human hepatocyte growth factor (HGF).
A host cell comprising:
- a first expression vector comprising a nucleic acid sequence encoding an immunoglobulin heavy chain comprising the amino acid sequence of SEQ ID NO:
- 187;
- and
- a second expression vector comprising a nucleic acid sequence encoding an immunoglobulin light chain comprising the amino acid sequence of SEQ ID NO:
- 197;
- wherein the immunoglobulin heavy chain and the immunoglobulin light chain together bind human hepatocyte growth factor (HGF).
Dependencies are inferred from the claim text.