Characterization of thermostable dna polymerase
AssigneeRoche Molecular Systems, Inc.
InventorLaura Jung
Life of this patent
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Abstract
Methods detecting covalent lysine modifications in DNA polymerases are provided. These methods are particularly useful in determining the extent and location of a lysine modification in a DNA polymerase.
Claims20
A method of detecting the number of covalent lysine modifications in a lysine-modified DNA polymerase, said method comprising
- (i) detecting the mass of the lysine-modified DNA polymerase at a modification stabilizing pH;
- and
- (ii) calculating the number of lysine modifications in the lysine-modified DNA polymerase by comparing the mass of the lysine-modified DNA polymerase to a polymerase without lysine modifications, wherein the number of lysine modifications is the difference of the mass of the lysine-modified DNA polymerase and the polymerase without lysine modifications divided by the mass of the lysine modification, thereby detecting the number of lysine modifications in the DNA polymerase.
The method of claim of 19, comprising detecting the relative amount of modification at said amino acid position, wherein the relative amount of modification is the amount of the modified lysine residue at said position divided by the sum of the amount of modified and unmodified lysine at said position, thereby detecting said relative amount of modification at said position.
Dependencies are inferred from the claim text.