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US 2014/0178904 A1Published application
Biochemical Markers of Red Blood Cell Storage and Toxicity
AssigneePuget Sound Blood Center
InventorJames Charles Zimring
Life of this patent
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Abstract
Compositions and methods for determining post-transfusion survival or toxicity of red blood cells and the suitability of red blood cell units for transfusion by measuring the levels of one or more markers in a red blood cell sample are provided.
Claims26
A method of determining post-transfusion survival of red blood cells (RBC) prior to transfusion, the method comprising the steps of:
- a) measuring the levels of one or more markers in a RBC sample selected from the group consisting of Cytidine, 5-Methylcytidine, N4-Actelcytidine, 9-HODE, 13-HODE, Prostaglandin E2, 5-HETE, 12-HETE, 15-HETE, arachdonic acid, 1-palmitoylglycerophosphoinositol, 1-stearoylglycerophosphoinositol, 13,14-dihydro-15-keto-prostaglandin E2, and the reticulocyte count of a blood donor at the time of donation;
- b) comparing the level of the one or more markers in the RBC sample with the level of the one or more markers present in a control sample,
- wherein a higher or lower level of the one or more markers in the RBC sample is indicative of a lower RBC storage quality.
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A method for determining RBC storage quality, the method comprising the steps of:
- obtaining a dataset associated with a sample of stored blood, wherein the dataset comprises at least one marker, selected from the group consisting of Cytidine, 5-Methylcytidine, N4-Actelcytidine, 9-HODE, 13-HODE, Prostaglandin E2, 5-HETE, 12-HETE, 15-HETE, arachdonic acid, 1-palmitoylglycerophosphoinositol, 1-stearoylglycerophosphoinositol, 13, 14-dihydro-15-keto-prostaglandin E2, and the reticulocyte count of a blood donor at the time of donation;
- analyzing the dataset to determine data for the at least one marker,
- wherein the data is positively correlated or negatively correlated with RBC storage quality of the sample of stored blood.
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A computer-implemented method for determining RBC storage quality, the method comprising the steps of:
- storing, in a storage memory, a dataset associated with a stored blood sample, wherein the dataset comprises data for at least one marker, selected from the group consisting of Cytidine, 5-Methylcytidine, N4-Actelcytidine, 9-HODE, 13-HODE, Prostaglandin E2, 5-HETE, 12-HETE, 15-HETE, arachdonic acid, 1-palmitoylglycerophosphoinositol, 1-stearoylglycerophosphoinositol, 13,14-dihydro-15-keto-prostaglandin E2, and the reticulocyte count of a blood donor at the time of donation;
- and
- analyzing, by a computer processor, the dataset to determine the expression or activity levels of the at least one marker, wherein the expression or activity levels are positively correlated or negatively correlated with RBC storage quality.
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Dependencies are inferred from the claim text.